Mutation induction in cultured human cells after low-dose and low-dose-rate γ-ray irradiation: detection by LOH analysis

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  • UMEBAYASHI Yukihiro
    Advanced Development and Support Center, The Institute of Physical and Chemical Research
  • HONMA Masamitsu
    Division of Genetics and Mutagenesis, National Institute of Health Sciences
  • SUZUKI Masao
    International Space Radiation Laboratory, National Institute of Radiological Sciences
  • SUZUKI Hiromi
    Space Utilization Promotion Department, Japan Space Forum Department of Space Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences
  • SHIMAZU Toru
    Space Utilization Promotion Department, Japan Space Forum
  • ISHIOKA Noriaki
    Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency
  • IWAKI Masaya
    Advanced Development and Support Center, The Institute of Physical and Chemical Research
  • YATAGAI Fumio
    Advanced Development and Support Center, The Institute of Physical and Chemical Research

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タイトル別名
  • Mutation Induction in Cultured Human Cells after Low-dose and Low-dose-rate .GAMMA.-ray Irradiation: Detection by LOH Analysis
  • Mutation induction in cultured human cells after low dose and low dose rate g ray irradiation detection by LOH analysis

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To study the genetic effects of low-doses and low-dose-rate ionizing radiation (IR), human lymphoblastoid TK6 cells were exposed to 30 mGy of γ-rays at a dose-rate of 1.2 mGy/hr. The frequency of early mutations (EMs) in the thymidine kinase (TK) gene locus was determined to be 1.7 × 10-6, or 1.9-fold higher than the level seen in unirradated controls. These mutations were analyzed with a loss of heterozygosity (LOH) detection system, a methodology which has been shown to be sensitive to the effects of radiation. Among the 15 EMs observed after IR exposure, 8 were small interstitial-deletion events restricted to the TK gene locus. However, this specific type of event was not found in unirradiated controls. Although these results were observed under the limited conditions, they strongly suggest that the LOH detection system can be used for estimating the genetic effects of a low-dose IR exposure delivered at a low-dose-rate.<br>

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