Gene Expression Analyses of Human Mesenchymal Stem Cells Cultured in Osteogenic Differentiation Medium for 3, 7, 14 and 21 Days by Genome Focus DNA Microarray and Real-time PCR

1 Citations
  • TAIRA M.
    Department of Dental Materials Science and Technology
  • CHOSA Naoyuki
    Department of Biochemistry, Iwate Medical University School of Dentistry
  • SASAKI Kaori
    Department of Dental Materials Science and Technology
  • SAITOH Setsuo
    Department of Dental Materials Science and Technology
  • NEZU Takashi
    Department of Dental Materials Science and Technology
  • SATO Nobuko
    Department of Biochemistry, Iwate Medical University School of Dentistry
  • ARAKI Yoshima
    Department of Dental Materials Science and Technology

Search this article

Abstract

The purpose of this study was to evaluate gene expressions of human mesenchymal stem cells (hMSC) cultured in osteogenic differentiation medium (OM) which contained ascorbic acid, β-glycerophosphate and dexamethasone for 0 (control), 3, 5, 7, 14 and 21 days by 8.5k Genome Focus DNA microarray and real-time PCR. It was confirmed by the DNA microarray analysis that 327 genes of hMSC were significantly up-regulated by culture in OM especially at 14 and 21 days while 156 genes were down-regulated. Up-regulated genes included osteoblast-related genes such as secreted phosphoprotein 1 (osteopontin) gene and hypothetical protein expressed in osteoblast gene, along with angiogenesis-related genes and cell cycle arrest-related genes, while down-regulated genes contained stroma-related genes and keratin-related genes. Expressions of several osteogenic differentiation marker genes such as (down-regulated) osteonectin gene and (up-regulated) BMP2 gene were also evaluated by real-time PCR. Gene expression database identified here might contribute to dental tissue engineering.

Journal

  • J. Oral Tissue Eng.

    J. Oral Tissue Eng. 5 35-47, 2007

    Japanese Association of Regenerative Dentistry

Citations (1)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top