Release of heat shock proteins from human neuroblastoma cells exposed to acrylamide
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- Sumizawa Tomoyuki
- Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
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- Igisu Hideki
- Department of Environmental Toxicology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health
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抄録
When human neuroblastoma cells (SH-SY5Y) were exposed to 0.5 - 5 mM acrylamide for 18 hr, the levels of heat shock proteins (HSPs) of 90, 70 and 27 kDa (Hsp90, Hsp70, and Hsp27, respectively) were elevated in the incubation media depending on the dose of acrylamide whereas only the Hsp70 level increased within cells. U0126, a specific inhibitor of extracellular signal-regulated protein kinase kinase and a potent suppressor of the cytotoxicity of acrylamide, suppressed the increase in the levels of all HSPs in the incubation media but not their expression within cells. Total protein concentrations in the incubation media increased depending on the dose of acrylamide, and this increase was associated with the increasing number of bands detected by silver staining after SDS-polyacrylamide gel electrophoresis. One of the clearest bands was identified as Hsp90 by peptide mass fingerprinting. Thus, acrylamide causes release of proteins, including that of HSPs, from SH-SY5Y cells. HSP in extracellular fluid may be a good indicator of cytotoxicity of acrylamide.<br>
収録刊行物
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 33 (1), 117-122, 2008
一般社団法人 日本毒性学会
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詳細情報 詳細情報について
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- CRID
- 1390001204902439552
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- NII論文ID
- 110006596037
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- NII書誌ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL書誌ID
- 9382212
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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