Artificial Organ Made in vivo —Valve Leaflet of the Jellyfish Valve—

• KISHI Ayumi

  Graduate School of Medical Science, Kitasato University

• ISOYAMA Takashi

  Department of Biomedical Engineering, University of Tokyo

• SAITO Itsuro

  Research Center for Advanced Science and Technology, University of Tokyo

• KOUNO Akimasa

  Department of Biomedical Engineering, University of Tokyo

• ONO Toshiya

  Department of Biomedical Engineering, University of Tokyo

• SUGINO Ayaka

  Department of Biomedical Engineering, University of Tokyo

• MITSUMUNE Norihiko

  Research Center for Advanced Science and Technology, University of Tokyo

• YAMAGUCHI Sachiko

  Department of Biomedical Engineering, University of Tokyo

• SHI Wei

  Department of Biomedical Engineering, University of Tokyo

• INOUE Yusuke

  Department of Biomedical Engineering, University of Tokyo

• NAKAGAWA Hidemoto

  Research Center for Advanced Science and Technology, University of Tokyo

• ABE Yusuke

  Department of Biomedical Engineering, University of Tokyo

• IMACHI Kou

  TUBERO, Tohoku University

• NOSHIRO Makoto

  Graduate School of Medical Science, Kitasato University

Bibliographic Information

Other Title

• 生体内で作る人工臓器ジェリーフィッシュ弁・弁葉
• 生体内で作る人工臟器ジェリーフィッシュ弁・弁葉
• セイタイナイ デ ツクル ジンコウ ゾウキ ジェリーフィッシュベン ベンヨウ

Abstract

Artificial materials used for artificial organs have sufficient strength and durability. However, their biocompatibility has not been improved yet to the level of tissue-derived biomaterials. On the other hand, there were some problems in artificial organs made of tissue-derived biomaterials; difficulty of forming the desired shapes, insufficiency of strength and durability, and infection control. It is necessary to build arbitrarily-shaped artificial organs that have sufficient strength and durability with tissue-derived biomaterials. In this study, we developed an in-vivo method to make a circle valve leaflet of a jellyfish valve using an insert molding technique. The jellyfish valve is an artificial valve developed at the University of Tokyo, and consists of the valve leaflet and a valve seat. The three types of molds for valve leaflets with different thickness (0.5 mm, 1.0 mm, and 1.2 mm) were made with an acrylic resin. A velour cloth was put inside each mold to ensure strength and durability. Two sets of molds were built; one with only suspended cell and another with both suspended cell and tissue fragments. Three types of molds were implanted under the skin of a goat. The performance and durability of jellyfish valves with tissue-engineered valve leaflets were assessed using a mock circulation circuit after the molds were extracted from the goats and the valve leaflets were fixed with formalin. Moreover, hematoxylin-and-eosin stained sections were observed. The results demonstrated that the valve leaflets covered with connective tissues have sufficient performance and durability of more than one month. In conclusion, we made a tissue-engineered circle valve leaflet with enough strength and durability to be used as heart valves in vivo using the insert molding technique.

Journal

• Transactions of Japanese Society for Medical and Biological Engineering

  Transactions of Japanese Society for Medical and Biological Engineering 45 (4), 267-273, 2007

  Japanese Society for Medical and Biological Engineering

Keywords

• insert molding technique
• in vivo
• tissue
• jellyfish valve

Details

• CRID: 1390001205268414080
• NII Article ID: 110006614029
• NII Article ID: 130004494014
• NII Book ID: AA11633569
• DOI: 10.11239/jsmbe.45.267
• ISSN: 18814379
• ISSN: 1347443X
• NDL BIB ID: 9389178
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9389178
• Web Site: https://search.jamas.or.jp/link/ui/2008137106
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed