731 骨マトリックス内における骨細胞の変形観察(S08-2 細胞の構造とメカノバイオロジー(2),S08 細胞の構造とメカノバイオロジー)

• 伊藤 慎一

  京都大・院

• 田中 基嗣

  京都大・工

• 安達 泰治

  京都大・工

• 上岡 寛

  岡山大・医歯薬

• 山本 照子

  東北大・歯

• 北條 正樹

  京都大・工

書誌事項

タイトル別名

• 731 Observation of Deformation of Osteocytes in Bone Matrix

抄録

Osteocyte network system embedded in bone matrix is considered as one of the important elements in the mechanosensory system in bone remodeling. In order to understand the mechanosensory mechanism of osteocytes network, it is essential to evaluate mechanical stimulation to osteocyte network quantitatively as well as to observe biochemical response of osteocyte network to deformation of bone matrix. This study aimed to develop the method to evaluate osteocyte network deformation against bone matrix deformation. Osteocyte network in chick calvarial bone tissue was visualized by immunostaining of cell membrane using OB7.3. Osteocyte network displacement induced by localized deformation to bone matrix using a glass microneedle was observed. Analysis of osteocyte network displacement was fairly successful using the image-correlation method. Applicability of the method newly developed in this study was discussed in combination with biochemical response observation of change in intracellular calcium ion concentration using the fluorescent ratiometry method, in order to investigate the relation between macroscopic deformation of bone matrix and microscopic cellular response.

収録刊行物

• 年次大会講演論文集

  年次大会講演論文集 2006.5 (0), 261-262, 2006

  一般社団法人 日本機械学会

キーワード

• Osteocytes
• Bone matrix
• Mechanosensory mechanism
• Mechanical stimulus
• Cell biomechanics
• Deformation

詳細情報

• CRID: 1390282681038200704
• NII論文ID: 110006635530
• DOI: 10.1299/jsmemecjo.2006.5.0_261
• ISSN: 24331325
• Web Site: https://www.jstage.jst.go.jp/article/jsmemecjo/2006.5/0/2006.5_261/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可