732 単一の単離骨細胞における局所変形による力学刺激とカルシウム応答の関係(S08-2 細胞の構造とメカノバイオロジー(2),S08 細胞の構造とメカノバイオロジー)

• 青沼 有紀

  神戸大・院

• 田中 基嗣

  京都大・工

• 安達 泰治

  京都大・工

• 上岡 寛

  岡山大・医歯薬

• 山本 照子

  東北大・歯

• 矢野 澄雄

  神戸大・発達科学

• 北條 正樹

  京都大・工

書誌事項

タイトル別名

• 732 Relation between Mechanical Stimulation by Localized Deformation and Calcium Signaling Response in Single Isolated Osteocytes

抄録

The osteocyte is considered as a candidate of the mechanosensory cells in bone tissue. In order to understand the mechanosensory mechanism of osteocytes, it is essential to evaluate mechanical stimulation to osteocytes quantitatively as well as to observe biochemical response of osteocytes to mechanical stimulus. This study aimed to develop the method to evaluate and control mechanical stimulation to osteocytes. Microbeads coated with OB7.3 were attached to chick calvarial isolated osteocytes. Mechanical stimulus applied by pushing microbeads using a glass microneedle was successfully evaluated and controlled. Change in the intracellular Ca^<2+> concentration induced by localized mechanical stimulation by a microneedle was observed using a confocal laser-scanning microscope. Calcium signaling response was up-regulated by mechanical stimulation to proximal base of cell process, while it was not up-regulated by mechanical stimulation to cell body.

収録刊行物

• 年次大会講演論文集

  年次大会講演論文集 2006.5 (0), 263-264, 2006

  一般社団法人 日本機械学会

キーワード

• Osteocyte
• Mechanosensory Mechanism
• Mechanical Stimulus
• Cell Biomechanics
• Calcium Signaling Response

詳細情報

• CRID: 1390001206061491968
• NII論文ID: 110006635531
• DOI: 10.1299/jsmemecjo.2006.5.0_263
• ISSN: 24331325
• Web Site: https://www.jstage.jst.go.jp/article/jsmemecjo/2006.5/0/2006.5_263/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可