A Novel Monofunctional DNA Glycosylase Activity Against Thymine Glycol in Mouse Cell Nuclei

• YAMAMOTO Ryohei

  Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University

• AKIYAMA Miyuki

  Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University

• IDE Hiroshi

  Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University

• YAMAMOTO Kazuo

  Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University

• MATSUYAMA Satoshi

  Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University

• KUBO Kihei

  Department of Advanced Pathobiology, Graduate School of Life & Environmental Sciences, Osaka Prefecture University

抄録

Reactive oxygen species continuously oxidize DNA bases and threaten the genetic integrity. Thymine glycol (TG), one of the representative oxidized products, is repaired mainly by base excision repair (BER). In Escherichia coli, endonuclease III (Nth) and endonuclease VIII (Nei) are known to actively remove TG from DNA, and the homologs are well conserved in various organisms. These are bifunctional glycosylases, also associated with apurinic/apyrimidinic (AP) lyase activity. In the present study, a monofunctional TG-DNA glycosylase activity is shown to be one of the predominant nuclear activities present in some mouse tissues. By combining hypertonic extraction and column chromatography, we successfully separated the novel activity from majority of the bifunctional activities. Since it has been reported that mNTH1 may not be a dominant nuclear activity, the monofunctional glycosylase activity, together with mNEIL1, may be the major TG-DNA glycosylases in the mouse nucleus. The optimal reaction conditions for the monofunctional activity were found to be pH 7-8 and 100-150 mM KCl, and the activity was resistant to 20 mM EDTA. High monofunctional activity was detected in the spleen and stomach, while the level was significantly lower in the liver, suggesting that the contribution of the monofunctional activity is variable among organs.

収録刊行物

• Journal of Radiation Research

  Journal of Radiation Research 49 (3), 249-259, 2008

  Journal of Radiation Research 編集委員会

キーワード

• Thymine glycol
• Glycosylase
• Mouse/BER
• BER

詳細情報

• CRID: 1390282680193858816
• NII論文ID: 110006666023
• NII書誌ID: AA00705792
• DOI: 10.1269/jrr.07100
• ISSN: 13499157
• ISSN: 04493060
• NDL書誌ID: 9490658
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9490658
• Web Site: http://academic.oup.com/jrr/article-pdf/49/3/249/2711298/jrr-49-249.pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10919045
• Web Site: https://search.jamas.or.jp/link/ui/2008326926
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可