P-72 海綿Theonella swinhoeiの細胞毒性ポリペプチドpolytheonamide BのNMRによる構造解析(ポスター発表の部)

• 濱田 季之

  東大院農:日本学術振興会

• 松永 茂樹

  東大院農

• 伏谷 伸宏

  東大院農

• 藤原 正子

  日本電子データム

• 藤田 憲一

  日本電子システムテクノロジー

書誌事項

タイトル別名

• P-72 STRUCTURE ELUCIDATION OF POLYTHEONAMIDE B, A HIGHLY CYTOTOXIC POLYPEPTIDE FROM THE MARINE SPONGE THEONELLA SWINHOEI, BY NMR SPECTROSCOPY

抄録

A highly cytotoxic polypeptide, polytheonamide B (1), has been isolated from the marine sponge Theonella swinhoei. The structure of polytheonamide B was determined, to be a linear 48-residues polypeptide with the N-terminus blocked by a carbamoyl group, by extensive 2D NMR experiments in DMSO-d_6. The absolute configuration of each amino acid residue was determined by chiral chromatographies of fragment peptides obtained by partial acid hydrolysis of polytheonamide B. Amino acid residues in polytheonamide B have alternating D- and L-configuration. The NMR spectra in CD_3OH/CDCl_3 (1:1) indicated that polytheonamide B adopted certain secondary structure. Conformation analysis was carried out by distance geometry calculations, by DADAS90 program, using NMR parameters obtained in CD_3OH/CDCl_3 (1:1), i.e., a total of 378 distance constraints (160 intra-residue, 96 sequential, 80 long-range NOEs, and 42 hydrogen bonds) and 48 dihedral angle constraints. The calculated structure fit a right-handed parallel β-helix structure, which was proposed for the structure of a pore-forming peptide, gramicidin A.

収録刊行物

• 天然有機化合物討論会講演要旨集

  天然有機化合物討論会講演要旨集 37 (0), 695-700, 1995

  天然有機化合物討論会実行委員会

詳細情報

• CRID: 1390001206078129408
• NII論文ID: 110006679493
• DOI: 10.24496/tennenyuki.37.0_695
• ISSN: 24331856
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可