Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR
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- Fukui Youhei FUKUI YOUHEI
- Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
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- Sawabe Tomoo SAWABE TOMOO
- Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
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Author(s)
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- Fukui Youhei FUKUI YOUHEI
- Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
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- Sawabe Tomoo SAWABE TOMOO
- Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University
Abstract
<i>Vibrio harveyi</i> cause mass mortalities of cultured marine fish. To address the ecology of <i>V. harveyi</i> in aquaculture, intensive monitoring is needed. We first optimized a quantitative real-time PCR method to determine <i>V. harveyi</i> abundance. The designed TaqMan probe and primers based on the 16S rRNA gene were specific at 68°C of annealing and extension. Furthermore, the method using a chelating resin method was able to enumerate 1.7×10<sup>2</sup> CFU/ml in breeding seawater at an abalone farm. This method represents a good tool for monitoring the ecology of <i>V. harveyi</i> in marine environments within 5 h.<br>
Journal
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- Microbes and Environments
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Microbes and Environments 23(2), 172-176, 2008-06-01
Japanese Society of Microbial Ecology
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