Rapid Detection of Vibrio harveyi in Seawater by Real-Time PCR

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Author(s)

Abstract

<i>Vibrio harveyi</i> cause mass mortalities of cultured marine fish. To address the ecology of <i>V. harveyi</i> in aquaculture, intensive monitoring is needed. We first optimized a quantitative real-time PCR method to determine <i>V. harveyi</i> abundance. The designed TaqMan probe and primers based on the 16S rRNA gene were specific at 68°C of annealing and extension. Furthermore, the method using a chelating resin method was able to enumerate 1.7×10<sup>2</sup> CFU/ml in breeding seawater at an abalone farm. This method represents a good tool for monitoring the ecology of <i>V. harveyi</i> in marine environments within 5 h.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 23(2), 172-176, 2008-06-01

    Japanese Society of Microbial Ecology

References:  16

Cited by:  2

Codes

  • NII Article ID (NAID)
    110006684370
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13426311
  • NDL Article ID
    9510944
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z54-J644
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE 
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