Silencing of CPD Photolyase Makes Arabidopsis Hypersensitive and Hypermutable in Response to UV-B Radiation

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Abstract

Plants are exposed to solar ultraviolet radiation (UV), which has deleterious effects on plant growth, development and physiology. Cyclobutane pyrimidine dimers (CPDs) are a major form of UV-induced DNA damage. It is conceivable that the reversal of CPDs is important for the reduction of lethal and mutagenic effects. Photoreactivation catalyzed by CPD photolyase is an efficient CPD repair system with a mechanism dependent on UV-A/visible light, which is contained in solar radiation. Photoreactivation presumably functions to protect plants from solar UV. We generated a CPD photolyase knock-down in <i>Arabidopsis thaliana</i> by RNA interference (RNAi) to investigate the role of CPD photorepair for protection of plants from solar UV. These knock-down lines exhibited hypersensitivity to UV-B and an increased occurrence of mutation induced by UV-B radiation compared with <i>Arabidopsis</i> proficient in CPD photolyase. Mutations induced by UV-B were determined by an <i>rpsL</i> mutation assay system. G:C to A:T transitions were frequently observed in CPD photolyase knock-down lines at the site of dipyrimidine sequences. A high incidence of frameshifts was observed in irradiated knock-down lines. These results indicate that CPD photoreactivation plays an important role for UV resistance of <i>Arabidopsis</i> and suppression of UV-induced mutagenesis.<br>

Journal

  • Genes and Environment

    Genes and Environment 30(2), 53-61, 2008-05-20

    The Environmental Mutagen Society of Japan

References:  57

Codes

  • NII Article ID (NAID)
    110006781782
  • NII NACSIS-CAT ID (NCID)
    AA1212552X
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    18807046
  • NDL Article ID
    9519348
  • NDL Source Classification
    ZR1(科学技術--生物学)
  • NDL Call No.
    Z78-A397
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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