Genetic Divergence and Identification of Two Controversial Lanternfishes (Actinopterygii: Myctophidae: Diaphus) Based on Mitochondrial Cytochrome b Sequences and PCR-RFLP Analysis Genetic Divergence and Identification of Two Controversial Lanternfishes (Actinopterygii: Myctophidae: Diaphus) Based on Mitochondrial Cytochrome b Sequences and PCR-RFLP Analysis

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Author(s)

Abstract

To resolve the controversial taxonomic status of two closely-related lanternfishes, Diaphus perspicillatus (Ogilby, 1898) and D.gigas Gilbert, 1913, mitochondrial cytochrome b (cyt b) gene sequences of representative individuals were obtained, and specifically-diagnostic restriction sites investigated. In the 5'-half nucleotide sequences of the cyt b gene (594 bp), a relatively high level of nucleotide substitution between the species (8.2-8.7%), together with very low intraspecific variation (<0.86%), was observed, which clearly indicates the two morphs to be specifically distinct. Previous hypotheses regarding gigantism of D.gigas and suspicions regarding the synonymy of the two species are rejected. The nucleotide sequences and practical restriction enzyme assay indicate that any one of four restriction endonucleases (Fok I, Hae III, Nla IV, and Rsa I) can unambiguously discriminate between the two species. Based on specimens identified by molecular analysis, eye diameter and gill raker count were found to be good diagnostic morphological characters.

Journal

  • Species Diversity

    Species Diversity 10(4), 289-299, 2005

    The Japanese Society of Systematic Zoology

References:  22

Cited by:  1

Codes

  • NII Article ID (NAID)
    110006794394
  • NII NACSIS-CAT ID (NCID)
    AA11114903
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13421670
  • NDL Article ID
    7756484
  • NDL Source Classification
    ZR4(科学技術--生物学--動物)
  • NDL Call No.
    Z53-W570
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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