Effects of Locally Targeted Heavy-ion and Laser Microbeam on Root Hydrotropism in Arabidopsis thaliana

Author(s)

Abstract

Classical studies on root hydrotropism have hypothesized the importance of columella cells as well as the <i>de novo</i> gene expression, such as auxin-inducible gene, at the elongation zone in hydrotropism; however, there has been no confirmation that columella cells or auxin-mediated signaling in the elongation zone are necessary for hydrotropism. We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporarily suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed <i>de novo</i> expression of <i>INDOLE ACETIC ACID 5</i> gene, but not <i>MIZU-KUSSEI1</i> gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that <i>de novo</i> gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.

Journal

  • Journal of Radiation Research

    Journal of Radiation Research 49(4), 373-379, 2008-07-15

    Journal of Radiation Research Editorial Committee

References:  21

Cited by:  2

Codes

  • NII Article ID (NAID)
    110006820176
  • NII NACSIS-CAT ID (NCID)
    AA00705792
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    04493060
  • NDL Article ID
    9577296
  • NDL Source Classification
    ZS45(科学技術--医学--放射線医学)
  • NDL Call No.
    Z53-P232
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE  NDL-Digital 
Page Top