Effects of Locally Targeted Heavy-ion and Laser Microbeam on Root Hydrotropism in Arabidopsis thaliana

• MIYAZAWA Yutaka

  Graduate School of Life Sciences, Tohoku University

• SAKASHITA Tetsuya

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA)

• FUNAYAMA Tomoo

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA)

• HAMADA Nobuyuki

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA) Department of Quantum Biology, and The 21st Century Center of Excellence (COE) Program for Biomedical Research Using Accelerator Technology, Gunma University, Graduate School of Medicine

• NEGISHI Hiroshi

  Graduate School of Life Sciences, Tohoku University

• KOBAYASHI Akie

  Graduate School of Life Sciences, Tohoku University

• KANEYASU Tomoko

  Graduate School of Life Sciences, Tohoku University

• OOBA Atsushi

  Graduate School of Life Sciences, Tohoku University

• MOROHASHI Keita

  Graduate School of Life Sciences, Tohoku University

• KAKIZAKI Takehiko

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA)

• WADA Seiichi

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA) Department of Quantum Biology, and The 21st Century Center of Excellence (COE) Program for Biomedical Research Using Accelerator Technology, Gunma University, Graduate School of Medicine

• KOBAYASHI Yasuhiko

  Microbeam Radiation Biology Group, Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA) Department of Quantum Biology, and The 21st Century Center of Excellence (COE) Program for Biomedical Research Using Accelerator Technology, Gunma University, Graduate School of Medicine

• FUJII Nobuharu

  Graduate School of Life Sciences, Tohoku University

• TAKAHASHI Hideyuki

  Graduate School of Life Sciences, Tohoku University

Abstract

Classical studies on root hydrotropism have hypothesized the importance of columella cells as well as the de novo gene expression, such as auxin-inducible gene, at the elongation zone in hydrotropism; however, there has been no confirmation that columella cells or auxin-mediated signaling in the elongation zone are necessary for hydrotropism. We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporarily suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed de novo expression of INDOLE ACETIC ACID 5 gene, but not MIZU-KUSSEI1 gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that de novo gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.

Journal

• Journal of Radiation Research

  Journal of Radiation Research 49 (4), 373-379, 2008

  Journal of Radiation Research Editorial Committee

Keywords

• <i>Arabidopsis thaliana</i>
• Heavy-ion microbeam
• Root hydrotropism
• Laser ablation
• Radiosurgery

Details

• CRID: 1390001205216206848
• NII Article ID: 110006820176
• NII Book ID: AA00705792
• DOI: 10.1269/jrr.07131
• ISSN: 13499157
• ISSN: 04493060
• NDL BIB ID: 9577296
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9577296
• Web Site: http://academic.oup.com/jrr/article-pdf/49/4/373/6383129/jrr-49-373.pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10919069
• Web Site: https://search.jamas.or.jp/link/ui/2009045774
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • NDL-Digital
  • CiNii Articles
• Abstract License Flag: Disallowed