Effects of cryopreservation on bone marrow derived mesenchymal cells of a nonhuman primate.

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  • Tokumoto Sayaka
    Section of Orthopaedic and Spinal Surgery, Tokyo Medical and Dental University Graduate School Section of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University Graduate School,
  • Sotome Shinichi
    Section of Orthopaedic and Spinal Surgery, Tokyo Medical and Dental University Graduate School Section of Regenerative Therapeutics for Spine and Spinal Cord, Tokyo Medical and Dental University Graduate School
  • Torigoe Ichirou
    Section of Orthopaedic and Spinal Surgery, Tokyo Medical and Dental University Graduate School
  • Omura Ken
    Section of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University Graduate School, Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of tooth and Bone, Tokyo Medical and Dental University
  • Shinomiya Kenichi
    Section of Orthopaedic and Spinal Surgery, Tokyo Medical and Dental University Graduate School Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of tooth and Bone, Tokyo Medical and Dental University Core to Core Program for Advanced Bone and Joint Science, Japan Society for the Promotion of Science Tokyo Medical and Dental University

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Abstract

Bone marrow stromal cells (MSCs) have multi-lineage differentiation capabilities and are focused on as a cell source for various cell therapies. To facilitate the availability of MSCs, cryopreservation technique is one of the critical factors for the cell therapies. In this study, effects of cryopreservation on capabilities of MSCs derived from a nonhuman primate were tested, aimed at a clinical application for tissue-engineered bone reconstruction. Effects of cryopreservation on the MSCs’ adhesion rate, proliferation, and osteogenic differentiation in vitro were compared with non-cryopreserved MSCs. Bone formation capabilities were also tested using an extraskeletal bone induction model. The bone formation inducted by the combination of cryopreserved MSCs and an artificial bone scaffold was confirmed in all cases. The amount of bone formation at each case was irregular, but the results were suggested the possibility of cryopreserved MSCs on clinical use.

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