Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring

• NEZU Takashi

  Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry

• MASUYAMA Tomoyuki

  Department of Operative Dentistry and Endodontics, Iwate Medical University School of Dentistry

• SASAKI Kaori

  Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry

• SAITOH Setsuo

  Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry

• TAIRA Masayuki

  Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry

• ARAKI Yoshima

  Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry

Abstract

The adsorption behaviors of lysozyme on dentally related Au, SiO₂, and TiO₂ surfaces were investigated by a quartz crystal microbalance with dissipation monitoring (QCM-D) method. Frequency shifts indicated that while lysozyme (pI 11) was fairly adsorbed on the SiO₂ (pI 1.9) surface at both pH 3 and 7, it was adsorbed on TiO₂ (pI 6.3) surface only at pH 7. However, adsorption was disturbed by 50 mM NaCl. These results strongly suggested an electrostatic nature of the adsorption behavior. Though a large-scale adsorption of the lysozyme on Au sensor was pH-insensitive, softness of the adlayer as seen from the dissipation profile was pH-dependent, indicating an interaction of another type. With all the surfaces, the small dissipation change indicated a stiff lysozyme adlayer. Results of this study revealed that the controlled electrostatic interaction between the material surface and lysozyme might be a useful method for imparting antibacterial property to the dental materials.

Journal

• Dental Materials Journal

  Dental Materials Journal 27 (4), 573-580, 2008

  The Japanese Society for Dental Materials and Devices

Keywords

• Lysozyme
• Adsorption
• QCM-D

Details

• CRID: 1390282679692067072
• NII Article ID: 110006861868
• NII Book ID: AA10443149
• DOI: 10.4012/dmj.27.573
• COI: 1:CAS:528:DC%2BD1cXht1Gqsb7N
• ISSN: 18811361
• ISSN: 02874547
• PubMed: 18833772
• Web Site: https://www.jstage.jst.go.jp/article/dmj/27/4/27_4_573/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10745214
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • NDL-Digital
  • CiNii Articles
• Abstract License Flag: Disallowed