Identification of Novel Betaproteobacteria in a Succinate-Assimilating Population in Denitrifying Rice Paddy Soil by Using Stable Isotope Probing

• Saito Takayuki

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Ishii Satoshi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Otsuka Shigeto

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Nishiyama Masaya

• Senoo Keishi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

抄録

Rice paddy soil has been shown to have strong denitrifying activity. However, the microbial populations responsible for the denitrification have not been well characterized. In this study, we employed Stable Isotope Probing (SIP) to study succinate-assimilating denitrifiers in soil microcosms amended with nitrate and ¹³C-succinate. Microbial populations represented in ¹²C- and ¹³C-DNA fractions were different based on denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified 16S rRNA gene fragment. A nearly full-length 16S rRNA gene was also amplified, cloned, and sequenced from ¹³C-DNA fraction. Both PCR-DGGE and clone library analyses revealed that Burkholderiales and Rhodocyclales dominated the succinate-assimilating population in denitrifying soil after 24 h incubation. Among these, novel Betaproteobacteria, possibly within the order Rhodocyclales, represented 43% of the clones obtained. Nitrite reductase genes, nirS and nirK, were also amplified and cloned from the ¹³C-DNA fraction. While most nirK clones in this study were similar to the nirK sequences from Rhizobiales, a majority of the nirS clones were similar to the nirS sequences from Burkholderiales and Rhodocyclales, consistent with the 16S rRNA gene analysis. These groups of bacteria, including the novel Betaproteobacteria, may play an important role in denitrification in rice paddy soil.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 23 (3), 192-200, 2008

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• denitrification
• succinate-assimilating population
• stable isotope probing
• rice paddy soil
• <i>Rhodocyclales</i>

詳細情報

• CRID: 1390282679322242048
• NII論文ID: 110006873789
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.23.192
• COI: 1:STN:280:DC%2BC3MvpvFOmug%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 9623734
• PubMed: 21558708
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9623734
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/23/3/23_8510/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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