Quantitative Analysis of Vitamin B2 in Foods with a Handmade Fluorometer

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  • 自作蛍光光度計を用いた食品に含まれるビタミンB2の定量分析
  • ジサク ケイコウ コウドケイ オ モチイタ ショクヒン ニ フクマレル ビタミン B2 ノ テイリョウ ブンセキ

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Abstract

<p>Chemical quantitative analysis is becoming more and more important in science and environmental education. Because of this, many simple analytical devices have been invented by teachers limited by school budgetary constraints. We also developed a handmade fluorometer which can measure the concentration of riboflavin (vitamin B2) and reported it in a previous paper. With this initial device, however, it was hard to measure the concentration of riboflavin present in foods. Therefore, we improved on it in the following ways. (1) With the use of an OP amplifier, the electric current of a solar battery can be measured instead of the voltage. (2) With the use of a BP filter which covers the detector (solar battery), the influence by coexistence can be eliminated. Consequently, the content of riboflavin in foods (skim milk, instant noodles, cornflakes, nutrient tonics) was able to be measured quite accurately (within 10% error) compared with the contents written on the packages. Riboflavin, one of the essential nutrients, is not only studied in science education but also in home economics and physical education. This device can therefore be used in a number of educational fields.</p>

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