Immunohistochemical and Morphologic Basis for Glutamate Signaling in the Rat Stomach

• Iijima Junko

  Laboratory of Cytology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University

• Horie Sawa

  Laboratory of Anatomy & Cellular Biology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University Graduate School of Health Sciences, Kyorin University

• Hasegawa Rumi

  Laboratory of Anatomy & Cellular Biology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University

• Yasui Hideaki

  Laboratory of Cytology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University Graduate School of Health Sciences, Kyorin University

• Takami Shigeru

  Laboratory of Anatomy & Cellular Biology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University Graduate School of Health Sciences, Kyorin University

抄録

Physiologic studies conducted in rats have demonstrated that afferent fibers of the gastric branch of the vagus nerve increase their firing rate with the intragastric administration of the amino acid glutamate, and the increased firing rate is blocked by the depletion of serotonin (5-HT), administration of the blocker for the serotonin type-3 receptor (SR3), or nitric oxide synthase (NOS). To understand glutamate signaling in the gastric mucosa at the cellular level, we have been studying rats as an animal model using anatomic and immunohistochemical procedures. Our results have indicated that 5-HT-immunoreactive (ir) cells are present in the superficial part of the gastric mucosal epithelium and in the base of the fundic glands, whereas immunoreactivity for SR3 is localized in the neck and its vicinity of the fundic glands. Further, NOS1/neuronal NOS-ir cells with a bipolar shape are located in the lamina propria where a dense network of neuronal cells is present. These results suggest that complex cellular events take place during intragastric glutamate signaling.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 31 (10), 1838-1840, 2008

  公益社団法人 日本薬学会

キーワード

• serotonin receptor type 3
• nitric oxide synthase
• fundic gland
• rat

詳細情報

• CRID: 1390001204624204800
• NII論文ID: 110006936317
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.31.1838
• COI: 1:CAS:528:DC%2BD1cXhsVOjtbjL
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 9655235
• PubMed: 18827338
• Web Site: http://id.ndl.go.jp/bib/9655235
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9655235
• Web Site: http://www.jstage.jst.go.jp/article/bpb/31/10/31_10_1838/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009061786
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可