Molecular Typing of Sand Fly Species (Diptera, Psychodidae, Phlebotominae) from Areas Endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S Ribosomal RNA Gene

• TERAYAMA Yoshimi

  Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University

• KATO Hirotomo

  Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University

• GOMEZ Eduardo A.

  Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica

• UEZATO Hiroshi

  Department of Dermatology, Faculty of Medicine, University of the Ryukyus

• CALVOPIÑA Manuel

  Department of Parasitology, Kochi Medical School, Kochi University

• IWATA Hiroyuki

  Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University

• HASHIGUCHI Yoshihisa

  Department of Parasitology, Kochi Medical School, Kochi University

Bibliographic Information

Other Title

• エクアドルのリーシュマニア症流行地におけるサシチョウバエの遺伝子タイピング法の確立
• Parasitology: Molecular typing of sand fly species (Diptera, Psychodidae, Phlebotominae) from areas endemic for Leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene
• Molecular typing of sand fly species from areas endemic for leishmaniasis in Ecuador by PCR-RFLP of 18S ribosomal RNA gene

Abstract

Surveillance of the distribution of sand fly species is important for prediction of the risk and expansion of Leishmania infection in endemic and surrounding areas. In the present study, a simple and reliable method of typing New World Lutzomyia species circulating in endemic areas in Ecuador was established by using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique. PCR-RFLP of 18S ribosomal RNA (rRNA) genes with the restriction enzyme AfaI and subsequently HinfI successfully identified seven sand fly species in nine endemic areas in Ecuador. Although intraspecific genetic-diversity affecting the RFLP-patterns was detected in a species, the patterns were species specific. The method promises to be a powerful tool for the classification of New World Lutzomyia species.<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 70 (9), 907-913, 2008

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• <i>Lutzomyia</i>
• PCR-RFLP
• Sand fly
• typing
• 18S ribosomal RNA gene

Details

• CRID: 1390001206428742784
• NII Article ID: 110006946005
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.70.907
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 9654642
• Web Site: http://hdl.handle.net/10126/3290
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030762111
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9654642
• Web Site: http://www.jstage.jst.go.jp/article/jvms/70/9/70_9_907/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009003399
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed