In-vitro assessment of fabric structural composite scaffold applicable to tissue regeneration around implants

• Baba Shunsuke

  Department of Regenerative Medicine, Foundation for Biomedical Research and Innovation:Department of Fixed Prosthodontics and Occlusion, Osaka Dental University Department of Fixed Prosthodontics and Occlusion, Osaka Dental University

• Inoue Takeomi

  Department of Regenerative Medicine, Foundation for Biomedical Research and Innovation:Department of Fixed Prosthodontics and Occlusion, Osaka Dental University

• Tanaka Nobuo

  Department of Regenerative Medicine, Foundation for Biomedical Research and Innovation:Department of Fixed Prosthodontics and Occlusion, Osaka Dental University

• Hiwa Chiaki

  Core Research Team, Organization of Advanced Science and Technology, Kobe University

• Adachi Taiji

  Department of Mechanical Engineering and Science, Kyoto University

• Hojo Masaki

  Department of Mechanical Engineering and Science, Kyoto University

• Morikuni Hidetoshi

  Graduate School of Dentistry (Orthodontics), Osaka Dental University

• Nishiura Aki

  Department of Orthodontics, Osaka Dental University

• Matsumoto Naoyuki

  Department of Orthodontics, Osaka Dental University

• Kawazoe Takayoshi

  Department of Fixed Prosthodontics and Occlusion, Osaka Dental University

Abstract

Using tissue engineering techniques, we developed osteogenetic graft materials composed of autogenous mesenchymal stem cells (MSCs) harvested from bone marrow and platelet-rich plasma (PRP) prepared from the patient's blood. In order to improve predict-ability of implant and periodontal regenerative therapies, we devised a fabric structural composite scaffold that maintains space for the transplanted cells to regenerate tissue. For the purpose of conducting in vitro verification of the osteogenetic potential of the osteoblast-like cells injected into the woven fabric composite scaffold, these cells were incubated for an extended period of time on the scaffold and evaluations were conducted on the scaffold interface. The in vitro evaluations verified the capacity of human MSCs adhering to the scaffold to differentiate into osteoblast-like cells under the defined differentiation inducing conditions, leading to significant mineralization.<br> These results verify that the woven fabric composite scaffold, infused with gelled material formed with cultured osteogenetic cells and PRP, can be used as an osteogenetic graft material in bone regenerative therapy around implants. These materials now await further evaluation by animal trials.

Journal

• Journal of Osaka Dental University

  Journal of Osaka Dental University 42 (2), 107-112, 2008

  Osaka Odontological Society

Keywords

• Regenerative medicine
• Composite scaffold

Details

• CRID: 1390001204962328960
• NII Article ID: 110006983703
• NII Book ID: AA00704144
• DOI: 10.18905/jodu.42.2_107
• ISSN: 21896488
• ISSN: 04752058
• Web Site: https://search.jamas.or.jp/link/ui/2009158500
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed