Higher Concentrations of Fluoride Ions Dramatically Inhibit the Survival of Osteoblasts

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Author(s)

    • HIRATA Isao
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
    • OKAZAKI Masayuki
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

Abstract

To examine the effect of fluoride on the survival of osteoblasts, media containing various concentrations of fluoride, 0〜5.0×10<sup>-2</sup> mol/L, were prepared and mouse osteoblast MC3T3-E1 cells were cultured. At the same time, media containing 0〜5.0×10<sup>-2</sup> mol/L of chloride as an indicator of pH were also adjusted. Although osteoblasts grew well at fluoride levels below 1.0×10<sup>-3</sup> mol/L, osteoblast survival was dramatically inhibited over 5.0×10<sup>-3</sup> mol/L of fluoride despite the pH of the medium scarcely decreasing when compared with the control pH 7.81, and osteoblasts showing favorable survival with 2.0×10<sup>-2</sup> mol/L of chloride even at pH 6.81. Fluorospectrophotometric observation showed quite different features between control cells and at higher concentrations of fluoride. These results suggested that fluoride dramatically inhibits osteoblast growth at concentrations above 5.0×10<sup>-3</sup> mol/L in the medium solution.

Journal

  • Journal of Oral Tissue Engineering

    Journal of Oral Tissue Engineering 6(1), 3-8, 2008

    Japanese Association of Regenerative Dentistry

Codes

  • NII Article ID (NAID)
    110007004833
  • NII NACSIS-CAT ID (NCID)
    AA1195240X
  • Text Lang
    ENG
  • ISSN
    1348-9623
  • Data Source
    NII-ELS  J-STAGE 
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