Preparation of injectable 3D-formed β-tricalcium phosphate bead/alginate composite for bone tissue engineering

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Author(s)

    • MATSUNO Tomonori
    • Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
    • OMATA Kazuhiko
    • Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
    • YAMAUCHI YOSHITAKA
    • Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University
    • TABATA Yasuhiko
    • Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University
    • SATOH Tazuko
    • Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University

Abstract

A novel, injectable bone tissue engineering material was developed that consisted of β-tricalcium phosphate (β-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of β-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable β-TCP beads and alginate gel which were injectable <I>via</I> a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0 wt% alginate concentration exhibited a compressive strength of 69 kPa in dry conditions, which was significantly higher than that exhibited by 1.0 wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for <I>in vivo</I> experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering.

Journal

  • Dental Materials Journal

    Dental Materials Journal 27(6), 827-834, 2008-11-01

    The Japanese Society for Dental Materials and Devices

References:  31

Cited by:  1

Codes

  • NII Article ID (NAID)
    110007004989
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    02874547
  • Data Source
    CJP  CJPref  NII-ELS  J-STAGE  NDL-Digital 
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