Linkage between Light Microscopic Observations and Molecular Analysis by Single-Cell PCR for Ciliates

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Author(s)

Abstract

The molecular analysis of ciliates for identification and phylogeny is usually conducted through PCR amplification and DNA sequencing, with DNA extracted from a large number of cells. Therefore, the analysis of ciliates is limited to only those species that can be cultured. We propose a single-cell PCR procedure to overcome the difficulty in the analysis of unculturable species. The procedure has been tested on 6 <i>Stichotrichia</i> strains and uncultured <i>Levicoleps biwae</i> cells, targeting 18S rRNA gene sequences, after carrying out microscopic observations and obtaining photographic records. This procedure enables the systematic analysis of unculturable ciliate strains in natural environments by linking the morphology and genotype of a single cell.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 23(4), 356-359, 2008-12-01

    Japanese Society of Microbial Ecology

References:  16

Cited by:  3

Codes

  • NII Article ID (NAID)
    110007005111
  • NII NACSIS-CAT ID (NCID)
    AA11173196
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13426311
  • NDL Article ID
    9727649
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z54-J644
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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