Linkage between Light Microscopic Observations and Molecular Analysis by Single-Cell PCR for Ciliates

• Shimano Satoshi

  Environment Education Center, Miyagi University of Education

• Sanbe Mitsuo

  Environment Education Center, Miyagi University of Education

• Kasahara Yasuhiro

  Institute of Low Temperature Science, Hokkaido University

抄録

The molecular analysis of ciliates for identification and phylogeny is usually conducted through PCR amplification and DNA sequencing, with DNA extracted from a large number of cells. Therefore, the analysis of ciliates is limited to only those species that can be cultured. We propose a single-cell PCR procedure to overcome the difficulty in the analysis of unculturable species. The procedure has been tested on 6 Stichotrichia strains and uncultured Levicoleps biwae cells, targeting 18S rRNA gene sequences, after carrying out microscopic observations and obtaining photographic records. This procedure enables the systematic analysis of unculturable ciliate strains in natural environments by linking the morphology and genotype of a single cell.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 23 (4), 356-359, 2008

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• Ciliates
• single-cell PCR
• 18S rRNA gene
• uncultured cell
• light microscopic analysis

詳細情報

• CRID: 1390282679323030016
• NII論文ID: 110007005111
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me08532
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 9727649
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9727649
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/23/4/23_ME08532/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可