Development of a multiwalled carbon nanotube coated collagen dish

• TERADA Michiko

  Oral Diagnosis and Oral Medicine, Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University

• ABE Shigeaki

  Department of Biomedical, Dental Materials and Engineering, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• AKASAKA Tsukasa

  Department of Biomedical, Dental Materials and Engineering, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• UO Motohiro

  Department of Biomedical, Dental Materials and Engineering, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• KITAGAWA Yoshimasa

  Oral Diagnosis and Oral Medicine, Department of Oral Pathobiological Science, Graduate School of Dental Medicine, Hokkaido University

• WATARI Fumio

  Department of Biomedical, Dental Materials and Engineering, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

Abstract

Carbon nanotubes (CNTs) are one of the most interesting nanomaterials because of their excellent characteristics. In this study, a transparent CNTs coating for cell culture dishes was developed and its properties for cell culture were estimated. Carboxylated multiwalled carbon nanotubes (MWCNTs) were dispersed in aqueous sodium cholate solution and applied on a collagen type I-coated cell culture dish (cover glass). The dish surface was homogeneously covered by MWCNTs without aggregation. The MWCNT-coated dish was slightly gray and had good transparency, so conventional optical microscopic observation of the cells on the MWCNT-coated dish was possible. Rat osteoblast-like cells cultured on the MWCNT-coated dish showed slightly lower viability and proliferation compared to the collagen-coated dish. The cell adhesion on the MWCNT-coated dish was much higher than that on the collagen-coated dish. Therefore, MWCNT-coating for dishes will be a useful new material for cell culture.

Journal

• Dental Materials Journal

  Dental Materials Journal 28 (1), 82-88, 2009

  The Japanese Society for Dental Materials and Devices

Keywords

• Multiwalled carbon nanotubes (MWCNTs)
• Collagen
• Cell culture

Details

• CRID: 1390282679694403584
• NII Article ID: 110007041975
• NII Book ID: AA10443149
• DOI: 10.4012/dmj.28.82
• COI: 1:CAS:528:DC%2BD1MXks1Orsro%3D
• ISSN: 18811361
• ISSN: 02874547
• HANDLE: 2115/38742
• PubMed: 19280972
• Web Site: https://www.jstage.jst.go.jp/article/dmj/28/1/28_1_82/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10745288
• Web Site: https://search.jamas.or.jp/link/ui/2009200999
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • NDL-Digital
  • CiNii Articles
• Abstract License Flag: Disallowed