Comparative study of antioxidant activity and antiproliferative effect of hot water and ethanol extracts from the mushroom Inonotus obliquus

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Abstract

The medicinal mushroom Inonotus obliquus is a traditional and widely used multi-functional fungus. Hot water (50℃, 70℃, and 80℃) and ethanol crude extracts of I. obliquus were investigated for their antioxidant activity with superoxide dismutase (SOD) and (1,1-diphenyl-2-picryhydrazyl) (DPPH) radical-scavenging activity assays. We also investigated the antiproliferative effects and ability of the extracts to induce apoptosis in human colon cancer DLD-1 cells. Among the four extracts, the ethanol extract (EE) exhibited the strongest SOD-like activity and antiproliferative effect on DLD-1 cells, and exposure to the EE resulted in the induction of apoptosis, whereas no apoptosis was observed in DLD-1 cells exposed to the hot water extracts (HWEs). HWE at 70℃ (HWE70) exhibited the strongest DPPH radical-scavenging activity (EC50, 126μg/ml), whereas the EE showed the weakest activity (EC50, 224μg/ml). The different biological activities among the four extracts may be attributed to differences in their chemical composition, partially supported by polysaccharide, protein and phenolic content, and the ^1H-NMR spectra.

Journal

  • Journal of bioscience and bioengineering

    Journal of bioscience and bioengineering 107(1), 42-48, 2009-01-25

    The Society for Biotechnology, Japan

References:  39

Cited by:  1

Codes

  • NII Article ID (NAID)
    110007042034
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13891723
  • NDL Article ID
    9776011
  • NDL Source Classification
    ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
  • NDL Call No.
    Z53-S65
  • Data Source
    CJP  CJPref  NDL  NII-ELS  Crossref 
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