Protein Reconstitution Methods for Visualizing Biomolecular Function in Living Cells

• OZAWA Takeaki

  Department of Chemistry, School of Science, The University of Tokyo PRESTO, Japan Science and Technology Agency

Abstract

  One of the most challenging researches in current biology and medicinal chemistry is to understand how individual cellular molecules interact together in living cells. To visualize such molecules, genetically-encoded reporters have been used widely. The most common reporters are firefly luciferase, renilla luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. In this review, novel design of split GFP and split luciferase is described. The principle is based on reconstitution of the split-reporter fragments when they are brought together into close proximity. The reconstitution methods are used for screening organelle-localized proteins, imaging dynamics of nuclear proteins and mRNAs in living cells, and visualizing protease activities in living animals. These methods are generally applicable for imaging of complex cellular processes and evaluating chemical effects in living cells and animals.<br>

Journal

• YAKUGAKU ZASSHI

  YAKUGAKU ZASSHI 129 (3), 289-295, 2009-03-01

  The Pharmaceutical Society of Japan

Keywords

• green fluorescent protein
• luciferase
• mRNA
• mitochondria

Details

• CRID: 1390282681103693568
• NII Article ID: 110007123175
• NII Book ID: AN00284903
• DOI: 10.1248/yakushi.129.289
• ISSN: 13475231
• ISSN: 00316903
• NDL BIB ID: 10193709
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10193709
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/129/3/129_3_289/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009188993
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed