Protein reconstitution methods for visualizing biomolecular function in living cells (誌上シンポジウム) Protein Reconstitution Methods for Visualizing Biomolecular Function in Living Cells

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Author(s)

    • 小澤 岳昌 OZAWA Takeaki
    • 東京大学大学院理学系研究科化学専攻|科学技術振興機構PREST Department of Chemistry, School of Science, The University of Tokyo

Abstract

  One of the most challenging researches in current biology and medicinal chemistry is to understand how individual cellular molecules interact together in living cells. To visualize such molecules, genetically-encoded reporters have been used widely. The most common reporters are firefly luciferase, <i>renilla</i> luciferase, green fluorescent protein (GFP) and its variants with various spectral properties. In this review, novel design of split GFP and split luciferase is described. The principle is based on reconstitution of the split-reporter fragments when they are brought together into close proximity. The reconstitution methods are used for screening organelle-localized proteins, imaging dynamics of nuclear proteins and mRNAs in living cells, and visualizing protease activities in living animals. These methods are generally applicable for imaging of complex cellular processes and evaluating chemical effects in living cells and animals.<br>

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 129(3), 289-295, 2009-03-01

    The Pharmaceutical Society of Japan

References:  27

Codes

  • NII Article ID (NAID)
    110007123175
  • NII NACSIS-CAT ID (NCID)
    AN00284903
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    00316903
  • NDL Article ID
    10193709
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z19-411
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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