Possible involvement of proteinaceous and non-proteinaceous trehalase inhibitors in the regulation of hemolymph trehalose concentration in Bombyx mori

• Tatun Nujira

  Department of Biology, Faculty of Science, Chiang Mai University

• Singtripop Tippawan

  Department of Biology, Faculty of Science, Chiang Mai University

• Osugi Shingo

  Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University

• Nachiangmai Siriluck

  Department of Biology, Faculty of Science, Chiang Mai University

• Iwami Masafumi

  Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University

• Sakurai Sho

  Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University

抄録

The midgut of the silkworm Bombyx mori contains two types of trehalase: soluble and membrane-bound. The trehalose concentration in larval hemolymph remains substantially high through the feeding period of the last (fifth) larval instar, despite appreciable trehalase activity through this period. We found that B. mori larval hemolymph contains two types of trehalase inhibitor, a proteinaceous inhibitor (inhibitor-P) and an inhibitor that is extractable with methanol and ethanol (inhibitor-M). Both inhibit the two types of trehalase, but with different sensitivities. Inhibitor-P inhibited soluble trehalase (ST) more effectively than membrane-bound trehalase (MT), while inhibitor-M inhibited MT slightly more than ST. The inhibitory activity of inhibitor-M was high during the early to middle feeding period, followed by a sudden decrease during the late feeding period. Inhibitor-P activity increased throughout the feeding period, and markedly decreased 2 days after gut purge. Thus, the two inhibitors exhibited stage-specific changes in the hemolymph, indicating coordinated inhibitory activity. Therefore, trehalose concentration regulation in the hemolymph appears to be complex, consisting of at least two inhibitors and two types of trehalase.

収録刊行物

• Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ

  Ａｐｐｌｉｅｄ　Ｅｎｔｏｍｏｌｏｇｙ　ａｎｄ　Ｚｏｏｌｏｇｙ 44 (1), 85-94, 2009

  日本応用動物昆虫学会

キーワード

• Soluble trehalase
• membrane-bound trehalase
• trehalase inhibitor
• midgut
• <i>Bombyx mori</i>

詳細情報

• CRID: 1390001206242723840
• NII論文ID: 110007125705
• NII書誌ID: AA00543238
• DOI: 10.1303/aez.2009.85
• ISSN: 1347605X
• ISSN: 00036862
• NDL書誌ID: 10165147
• Web Site: http://id.ndl.go.jp/bib/10165147
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10165147
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可