ホタテ貝殻焼成粉末の殺菌および殺インフルエンザウイルス作用について  [in Japanese] Bactericidal and virucidal activities of heated scallop shell powders  [in Japanese]

Access this Article

Author(s)

    • 村田 亜悠美 MURATA Ayumi
    • 富山大学医学部看護学科感染看護学 Departments of Infedion Control Nusing, Faculty of Mecine, University of Toyama
    • 小尾 信子 OBI Nobuko
    • 富山大学医学部医学科和漢診療学 Departments of Oriental Medicine, Faculty of Mecine, University of Toyama
    • 宮原 龍郎 MIYAHARA Tatsuro
    • 富山大学医学部看護学科感染看護学 Departments of Infedion Control Nusing, Faculty of Mecine, University of Toyama
    • 落合 宏 OCHIAI Hiroshi
    • 富山大学医学部看護学科感染看護学 Departments of Infedion Control Nusing, Faculty of Mecine, University of Toyama

Abstract

ホタテ貝殻焼成粉末(HSSP)の殺菌および殺インフルエンザウイルス作用を検討した.微細HSSP(平均粒径3.5μm:f-HSSP)の0.15%生理食塩水懸濁液に5分接触させた場合,大腸菌の生残菌数は検出限界以下であったが,緑膿菌と黄色ブドウ球菌の生残率は,それぞれ3×10-3%と2.5%であり,大腸菌に最も強く殺菌作用を示した.f-HSSP,粗製HSSP(平均粒径18.4μm:c-HSSP),酸化カルシウムおよび水酸化カルシウムの0.15%生理食塩水懸濁液はほぼ同じpH(12.6~12.76)を示した.しかし,これら4試薬の大腸菌に対する経時的殺菌力を比較したところ,5分接触では全ての試薬においても生残率は検出限界以下であったものの,2分より短い接触時間域でf-HSSPが他3試薬より10~1000倍強い殺菌作用を示した.さらに,f-HSSP生理食塩水懸濁液は,濃度と接触時間依存的にインフルエンザウイルスA型PR8株とB型Sing株の感染性を失活させた.しかし,f-HSSP(37℃,30分接触)は少なくともA型PR8株の赤血球凝集活性には影響を与えなかった.0.75%生理食塩水懸濁液の殺インフルエンザウイルス作用を4試薬間で比較したところ,f-HSSPは短時間接触域で,c-HSSPよりおよそ1000倍強かったが,酸化カルシウムと水酸化カルシウムよりは弱い傾向を示した.これらの結果は,f-HSSPは強い大腸菌殺菌作用に加え,殺インフルエンザウイルス作用があることを示している.いずれにしても,このように少なくともc-HSSPより強力な抗微生物作用を有するf-HSSPは,感染看護の視点からも,様々な医療器具・看護器材の有用な天然資材と思われた.興味あることは,リン酸緩衝食塩水に懸濁すると,これらの作用は100倍から1000倍に減弱することであった.この知見に加え,4試薬間の生物活性の強さの違いの研究は今後の検討課題として残った.

We investigated bactericidal and virucidal activities of scallop shell powder (HSSP), of which main component is CaO possibly converting to Ca(OH)_2 in water. After 5-min contact with 0.15% saline suspension of fine HSSP (f-HSSP) with the mean particle diameter (mpd) of 3.5μm, survival rate of Escherichia (E) coli was uncountable, but these values of Pseudomonas aeruginosa and Staphylococcus aureus were 3×10^<-3>% and 2.5%, respectively, suggesting that E.coli shows the highest sensitivity to f-HSSP. Time-related bactericidal study, using 0.15% saline suspension of f-HSSP, crude HSSP (c-HSSP) with the mpd of 18.4μm, CaO and Ca(OH)_2, indicated that f-HSSP among these 4 reagents shows the strongest activity against E.coli especially in a shorter contact time range (less than 2 min), although pH values of suspensions were almost the same (12.6 to 12.76) in all reagents.Importantly, this study clearly demonstrated that saline suspension of f-HSSP shows virucidal activity against both influenza A/PR8 and B/Sing viruses in dose- and contact time-dependent manners, and 10-min contact with 0.75% f-HSSP induces a complete loss of infectivity of both viruses. Furthermore, treatment with 0.75% f-HSSP at 37℃ for 30 min did not affect hemagglutinating activity of A/PR8 virus. The virucidal activity of f-HSSP was also almost 1000-fold stronger than that of c-HSSP in a shorter contact time range, but seemed to beweaker than those of CaO and Ca(OH)_2 which could induce a complete loss of infectivity by as short as 5-min contact. Taking together, it is suggested that f-HSSP might be a useful natural product to prepare various medical and nursing devices from the point of view of infection control. Interestingly, bactericidal and virucidal activities of f-HSSP became 100 to 1000-fold weaker when it was suspended in the phosphate-buffered saline instead of saline. In addition to this finding, differences in biological activities among 4 reagents shown in this study remained to be solved.

Journal

  • The journal of the Nursing Society of University of Toyama

    The journal of the Nursing Society of University of Toyama 7(2), 39-49, 2008-03

    University of Toyama

Codes

  • NII Article ID (NAID)
    110007125877
  • NII NACSIS-CAT ID (NCID)
    AA12209357
  • Text Lang
    JPN
  • Article Type
    departmental bulletin paper
  • Journal Type
    大学紀要
  • ISSN
    1882191X
  • NDL Article ID
    9491633
  • NDL Source Classification
    ZS47(科学技術--医学--治療医学・看護学・漢方医学)
  • NDL Call No.
    Z74-B352
  • Data Source
    NDL  NII-ELS  IR 
Page Top