Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs

• Kume Teruyoshi

  Division of Cardiology, Kawasaki Medical School

• Kawamoto Takahiro

  Division of Cardiology, Kawasaki Medical School

• Okura Hiroyuki

  Division of Cardiology, Kawasaki Medical School

• Neishi Yoji

  Division of Cardiology, Kawasaki Medical School

• Hashimoto Ken

  Department of Physiology, Kawasaki Medical School

• Hayashida Akihiro

  Division of Cardiology, Kawasaki Medical School

• Watanabe Nozomi

  Division of Cardiology, Kawasaki Medical School

• Kanda Yukiko

  Division of Diabetes and Endocrine, Kawasaki Medical School

• Mochizuki Seiichi

  Department of Medical Engineering, Kawasaki Medical School

• Goto Masami

  Department of Medical Engineering, Kawasaki Medical School

• Yoshida Kiyoshi

  Division of Cardiology, Kawasaki Medical School

Abstract

Background: Direct measurement of plasma nitric oxide (NO) concentration is possible with a newly developed catheter-type sensor. Methods and Results: Adult mongrel dogs (n=5) were fed a high-fat diet (120 kcal · kg^-1 · day^-1) for 8 months, then endothelial function was assessed by the change in NO concentration induced by acetylcholine (ACh) (ΔNO). Simultaneously, average peak velocity (APV) was obtained by Doppler guide wire. Although fasting plasma glucose levels did not change after high-fat diet, fasting plasma insulin levels increased significantly (103±36 vs 106±27 mg/dl, P=0.89 and 0.15±0.15 vs 0.26±0.07 ng/ml, P=0.04, respectively). ACh-induced peak APV after high-fat feeding was not significantly different from that at baseline (ACh 0.1 μg/kg; 43±17 vs 51±7 cm/s, P=NS, ACh 0.4 μg/kg; 45±20 vs 47±16 cm/s, P=NS, respectively). The ΔNO was significantly smaller after high-fat diet than at baseline (ACh 0.1 μg/kg; 2.6±1.6 vs 1.0±0.5 nmol/L, P=0.03, ACh 0.4 μg/kg; 3.8±2.3 vs 1.8±1.1 nmol/L, P=0.04, respectively). Conclusions: In high-fat-diet-induced obese dogs NO production was impaired in the early stage when the coronary flow response to ACh may be preserved. (Circ J 2009; 73: 562 - 567)<br>

Journal

• Circulation Journal

  Circulation Journal 73 (3), 562-567, 2009

  The Japanese Circulation Society

Keywords

• Catheters
• Coronary circulation
• Metabolic syndrome
• Nitric oxide
• Obesity

Details

• CRID: 1390001205102936320
• NII Article ID: 110007126718
• NII Book ID: AA11591968
• DOI: 10.1253/circj.cj-08-0789
• ISSN: 13474820
• ISSN: 13469843
• Web Site: http://www.jstage.jst.go.jp/article/circj/73/3/73_CJ-08-0789/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009230918
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed