Important Regulatory Role of Activated Platelet-Derived Procoagulant Activity in the Propagation of Thrombi Formed Under Arterial Blood Flow Conditions
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- Tamura Noriko
- Department of Medicine, Tokai University School of Medicine
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- Kitajima Isao
- Department of Clinical Laboratory and Molecular Pathology, University of Toyama
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- Kawamura Yota
- Department of Medicine, Tokai University School of Medicine
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- Toda Eri
- Department of Medicine, Tokai University School of Medicine
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- Eguchi Yu
- Department of Medicine, Tokai University School of Medicine
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- Ishida Hideyuki
- Department of Basic Science, Tokai University School of Medicine
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- Goto Shinya
- Department of Medicine, Tokai University School of Medicine
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Abstract
Background: The functional links between the activation of platelets and the coagulation system have not been clarified. Methods and Results: Immobilized collagen fibrils were perfused with human blood containing fluoresceinated platelets in the presence of various concentrations of thrombin inhibitor. Coagulant activity around platelet thrombi was detected using a FITC-conjugated antibody against the fibrin monomer complex (F-405). Intra-cytosolic calcium ion concentrations ([Ca2+]i) in individual platelets and the volume of thrombi were detected with an ultrafast confocal laser microscope equipped with a piezo-motor control unit. The volume of platelet thrombi formed after 8 min of blood perfusion in the presence of 10, 25, 50, and 100 μmol/L argatroban was 7.69±0.46 μm3, 6.61±1.96 μm3, 3.63±1.54 μm3, and 1.67±0.75 μm3, respectively. There was a positive correlation between the volume of platelet thrombi and the amount of fibrin monomer complex produced around them. The [Ca2+]i of the platelets forming the thrombi oscillated between a minimum of 92.0±57.4 nmol/L, 120.1±68.1 nmol/L, and a maximum of 217.6±131.5 nmol/L, 367.6±189.1 nmol/L, respectively, in the presence of 100 and 10 μmol/L argatroban. Conclusions: The results suggest a crucial role of coagulant activity in both the generation of fibrin and the growth of platelet thrombi. (Circ J 2009; 73: 540 - 548)<br>
Journal
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- Circulation Journal
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Circulation Journal 73 (3), 540-548, 2009
The Japanese Circulation Society
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Details 詳細情報について
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- CRID
- 1390001205102915968
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- NII Article ID
- 110007126721
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- NII Book ID
- AA11591968
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- COI
- 1:CAS:528:DC%2BD1MXjvFOqsro%3D
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- ISSN
- 13474820
- 13469843
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- PubMed
- 19179771
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed