象牙質-歯髄境界部における侵入細菌の局在性に関する免疫組織学的研究  [in Japanese] An Immunohistological Study on the Localization of Selected Bacteria in Advancing Bacterial Front of Human Deep-carious Teeth  [in Japanese]

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Author(s)

    • 中西 正 NAKANISHI Tadashi
    • 徳島大学大学院ヘルスバイオサイエンス研究部発達予防医歯学部門健康長寿歯科学講座歯科保存学分野 Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School
    • 尾崎 和美 OZAKI Kazumi
    • 徳島大学歯学部口腔保健学科口腔保健支援学講座 Department of Oral Health Care Promotion, School of Oral Health and Welfare, The University of Tokushima
    • 松尾 敬志 MATSUO Takashi
    • 徳島大学大学院ヘルスバイオサイエンス研究部発達予防医歯学部門健康長寿歯科学講座歯科保存学分野 Department of Conservative Dentistry, Institute of Health Biosciences, The University of Tokushima Graduate School

Abstract

う蝕の進行に伴い歯髄炎が惹起された歯髄において,う蝕病巣の最前線部に存在する細菌の実態を知ることは,歯髄炎の病態を理解するだけでなく治療を行ううえでも重要である.今回,歯髄炎を伴う深在う蝕を有するヒト抜去歯の連続切片を用いて,象牙質-歯髄境界部に存在するう蝕関連細菌を同定し,その局在を明らかにすることを目的とした.歯髄に近接あるいは到達する深在う蝕ならびに不可逆性歯髄炎の臨床症状を有し,臨床的に抜歯適応と診断された抜去智歯のうち,Brown-Brenn染色にて修復象牙質あるいは歯髄組織内へ細菌の侵入が認められた14試料を被験対象とした.侵入細菌の同定は,6種のう蝕関連細菌に対する抗血清を用いた酵素抗体法にて行った.今回検索したすべての試料中の修復象牙質内に,Lactobacillus plantarumが認められた.Streptococcus mutans, Micromonas micros, Lactobacillus caseiについても,修復象牙質内での細菌検出率は約90%であった.これら4種の細菌種は,細菌侵入が歯髄腔に到達している試料の歯髄組織においても検出された.一方,Actinomyces viscosusは約40%,Streptococcus sanguinisは約20%程度の検出率であった.以上の結果より,臨床的に不可逆性歯髄炎の症状を呈する深在う蝕歯の,露髄部付近における細菌侵入の実態の一端が明らかとなった.

The characterization of advancing bacterial front of carious lesions is important not only for the pathogenesis of pulpitis, but also for the treatment of pulpal pathosis. We immunohistologically examined the prevalence and localization of selected bacteria in human deep-carious teeth. Fourteen specimens were obtained from extracted carious third molars that showed bacterial invasion in reparative dentin and/or dental pulp. The distribution of bacterial penetration was detected nonspecifically by Brown-Brenn stain and specifically enzyme-labeled antibody method with specific rabbit antisera against six bacteria. All of the samples showed the invasion of Lactobacillus plantarum in the reparative dentin. About 90% of the examined teeth showed the invasion of Streptococcus mutans, Micromonas micros and Lactobacillus casei in the reparative dentin. These four bacteria were also detected in the dental pulp with carious exposure. In contrast, the detection frequencies of Actinomyces viscosus and Streptococcus sanguinis in reparative dentin were about 40% and 20%, respectively. The present study revealed the features of advancing bacterial front of deep-carious teeth with irreversible pulpitis.

Journal

  • The Japanese Journal of Conservative Dentistry

    The Japanese Journal of Conservative Dentistry 51(1), 63-71, 2008

    The Japanese Society of Conservative Dentistry

References:  22

Cited by:  2

Codes

  • NII Article ID (NAID)
    110007151216
  • NII NACSIS-CAT ID (NCID)
    AN00191201
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0387-2343
  • Data Source
    CJP  CJPref  NII-ELS  J-STAGE 
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