Intracellular and Extracellular Redox Environments Surrounding Redox-Sensitive Contrast Agents under Oxidative Atmosphere
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- Okajo Aya
- Department of Physical Chemistry, Showa Pharmaceutical University
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- Ui Iori
- Department of Physical Chemistry, Showa Pharmaceutical University
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- Manda Sushma
- Radiation Modifier Research Team, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
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- Nakanishi Ikuo
- Radiation Modifier Research Team, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
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- Matsumoto Ken-ichiro
- Radiation Modifier Research Team, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
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- Anzai Kazunori
- Radiation Modifier Research Team, Heavy-Ion Radiobiology Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences
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- Endo Kazutoyo
- Department of Physical Chemistry, Showa Pharmaceutical University
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Abstract
In vivo redox reactions of nitroxyl contrast agents in bile and blood under an oxidative atmosphere were investigated using normal healthy Wistar rats. Differences in intracellular and extracellular volumes in redox environments are discussed. Pharmacokinetic profiles of two nitroxyl contrast agents, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (carbamoyl-PROXYL), 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL), in bile and blood were monitored by an electron paramagnetic resonance spectrometer when the rat was breathing 100% O2 or was subcutaneously administrated 0.2 mmol/kg body weight of ferric citrate. Re-oxidation of hydroxylamines to nitroxyl radicals was caused in bile under 100% O2 breathing, but not in blood. The administration of ferric citrate caused marked re-oxidation in bile, but a slight reduction in blood. Tissue H2O2 level may partly play a role in the intracellular re-oxidation process. Tissue Fe3+ concentration can work more effectively for the intracellular re-oxidation of hydroxylamines. The intracellular environment is susceptible to oxidation compared with the extracellular environment under conditions such as 100% O2 breathing or iron overload.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 32 (4), 535-541, 2009
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204624427392
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- NII Article ID
- 110007160706
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 10194136
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed