ツェツェバエ(Glossina morsitans morsitans)のトリプシン,キモトリプシン様遺伝子の発現解析 Characterization of trypsin-and chymotrypsin-like genes in the midgut of the tsetse fly Glossina morsitans morsitans (Diptera: Glossinidae)

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Abstract

ツェツェバエ(Glossina morsitans morsitans)は,アフリカサハラ砂漠以南に生息する眠り病の媒介昆虫である.ツェツェバエ中腸ESTから得られたセリンプロテアーゼ遺伝子群のうちGmmtry1とGmmchy1の2つの遺伝子を解析した.両遺伝子ともセリンプロテアーゼに特異的なヒスチジン(H),アスパラギン酸(D),セリン(S)残基を有していることが示された.Gmmtry1とGmmchy1遺伝子のmRNAは中腸後部で特異的に発現し,Gmmchy1の発現は,吸血によって誘導され約48時間後にピークに達した.さらに定量PCR法によりGmmchy1の発現がトリパノソーマ原虫の感染初期において上昇することが判明した.以上の結果から,Gmmchy1はツェツェバエ中腸内でのトリパノソーマ感染初期における原虫排除に関わっている可能性が示唆された.

Putative serine protease genes including trypsin and chymotrypsin were identified in the tsetse fly Glossina morsitans morsitans during an EST project. Trypsin and chymotrypsin are major digestive enzymes involved in the parasite-host interactions of vector insects. In this study, two genes designated Gmmtry1 and Gmmchy1 were further characterized during blood digestion and in responses against parasite infection. Both Gmmtry1 and Gmmchy1 mRNA was expressed at high levels in the posterior region of the midgut. Gmmchy1 transcript increased following an infectious blood meal suggesting chymotrypsin is regulated by the presence of the trypanosome during the early establishment of the parasite infection in the midgut. The possible roles of trypsin and chymotrypsin proteases in determining the refractoriness of the tsetse fly against trypanosomes is also discussed.

Journal

  • Medical Entomology and Zoology

    Medical Entomology and Zoology 60(1), 13-22, 2009

    The Japan Society of Medical Entomology and Zoology

References:  41

Codes

  • NII Article ID (NAID)
    110007226357
  • NII NACSIS-CAT ID (NCID)
    AN00021948
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    0424-7086
  • NDL Article ID
    10329382
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z19-47
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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