Label-free Detection of Allergens in Milk Using a Metallic Mesh Sensor
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- Yoshida Hisa
- Tohoku University
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- Ogawa Yuichi
- Tohoku University
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- Hayashi Shin'ichiro
- Tohoku University RIKEN
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- Otani Chiko
- RIKEN
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- Kawase Kodo
- Tohoku University RIKEN Nagoya University
Bibliographic Information
- Other Title
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- 金属メッシュセンサによる牛乳中アレルゲンのラベルフリー検出
- キンゾク メッシュセンサ ニ ヨル ギュウニュウ チュウ アレルゲン ノ ラベルフリー ケンシュツ
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Abstract
We propose a new label-free allergen sensing technique using a metallic mesh and a nitrocellulose membrane, which is generally used for fixation of protein. Our sensing method relies on a change in the transmittance of terahertz (THz) radiation passed through the metallic mesh on which a sample was placed. Label-free detection using THz radiation is a new technique in biomedicine that enables us to make easier and faster food allergen inspections. By using this technique, we demonstrated detecting the interaction between a streptavidin - biotin and α-casein- anti α-casein antibody on each membrane. The streptavidin - biotin interaction could be detected with 1.6 - 5.0 ng/mm2 streptavidin. The possibility of detecting the α-casein - anti α-casein antibody interaction was shown with 9.0 ng/mm2 α-casein. These results indicate that our sensing system is sensitive enough to detect allergens in food.
Journal
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- JOURNAL OF THE ILLUMINATING ENGINEERING INSTITUTE OF JAPAN
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JOURNAL OF THE ILLUMINATING ENGINEERING INSTITUTE OF JAPAN 93 (8A), 487-491, 2009
The Illuminating Engineering Institute of Japan
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Details 詳細情報について
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- CRID
- 1390001204077438208
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- NII Article ID
- 110007360153
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- NII Book ID
- AN00268860
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- ISSN
- 1349838X
- 21851506
- 00192341
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- NDL BIB ID
- 10396417
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed