8. タバコ培養細胞の液体窒素保存(平成5年度第39回凍結及び乾燥研究会研究報告)

• 西松 慶子

  大阪大学工学部応用生物工学科

• 田村 理

  大阪大学工学部応用生物工学科

• 関根 政実

  大阪大学工学部応用生物工学科

• 高野 光男

  大阪大学工学部応用生物工学科

書誌事項

タイトル別名

• 8. Vitrification of Tobacco BY2 Cells(Papers presented at the 39th Annual Meeting)

抄録

Cell suspension of Nicotiana tabacum L. cv. BY2 was cultured with a modified LS medium. The cells were examined to vitrify through two methods. The 7-days culture was replaced in the medium with 1M sucrose and cultured for 1 day. In the method 1, the cells were vitrified with PVS2 (by SAKAI) in liquid nitrogen. In the method 2, the cells were transferred to the medium containing 2M glycerol plus 0.4M sorbitol, and then to that containing 4.5M glycerol plus 1.46M sorbitol successively. Cells in the concentrated medium were vitrified similarly to the method 1. Plasmolysis of the cells was observed in both methods, but more mildly in the method 2. The ratio of heat of devitrification to that of thawing was 0.82 or 0.67 in the two methods, respectively. The thawed cells in both methods were hard to grow on the solid medium. Only the growth was observed on a solid medium through nursing culture plate of the cells from methed 2.

収録刊行物

• 凍結および乾燥研究会会誌

  凍結および乾燥研究会会誌 39 (0), 48-50, 1993

  低温生物工学会

キーワード

• タバコ培養細胞
• ガラス化
• ナース培養
• 原形質分離
• ガラス化率

詳細情報

• CRID: 1390001205087620736
• NII論文ID: 110007368529
• DOI: 10.20585/touketsukansokaishi.39.0_48
• ISSN: 24329916
• ISSN: 02888297
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可