Capillary gel electrophoresis for ligase detection reaction products

• Hashimoto Masahiko

• Kamigori Jun

• Tsukagoshi Kazuhiko

Bibliographic Information

Other Title

• キャピラリーゲル電気泳動によるリガーゼ検出反応生成物の分析
• キャピラリーゲル デンキ エイドウ 二ヨル リガーゼ ケンシュツ ハンノウ セイセイブツ ノ ブンセキ
• キャピラリー ゲル デンキ エイドウ ニ ヨル リガーゼ ケンシュツ ハンノウ セイセイブツ ノ ブンセキ

Abstract

One technique that can distinguish low-abundant mutant DNA from wild-type DNA is the ligase detection reaction (LDR) coupled to a primary polymerase chain reaction (PCR). The LDR products obtained by the PCR/LDR assay can be analyzed in a variety of fashions such as microarray and slab gel electrophoresis. In the present work, we applied capillary gel electrophoresis (CGE) with fluorescence detection (FL) (CGE-FL) for analyzing the LDR products. Polyethylene oxide (PEO) polymer solution containing SYBR Gold, which could bind to a single strand DNA, was used as a separation matrix in the CGE-FL system. The LDR products could successfully be separated from other DNA fragments such as LDR primers and template, enabling the determination of the single base substitutions on codon 12 of K-ras gene.

Journal

• 同志社大学理工学研究報告

  同志社大学理工学研究報告 50 (4), 6-12, 2010-01-31

  Science and Engineering Research Institute of Doshisha University

Keywords

• capillary gel electrophoresis
• polymer solution
• point mutation
• ligase detection reaction

Details

• CRID: 1390009224913385984
• NII Article ID: 110007506069
• NII Book ID: AN00165868
• DOI: 10.14988/pa.2017.0000012060
• NDL BIB ID: 10582416
• ISSN: 00368172
• Web Site: https://doshisha.repo.nii.ac.jp/records/20966
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10582416
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed