Detection of Label-Free T4-DNA Molecules Using SPR Technique

• OKUNO Hiroki

  the Department of Intelligent Mechanical Systems Engineering, Faculty of Engineering, Kagawa University

• NISHIOKA Ayami

  the Department of Intelligent Mechanical Systems Engineering, Faculty of Engineering, Kagawa University

• HOSOGI Maho

  the Department of Intelligent Mechanical Systems Engineering, Faculty of Engineering, Kagawa University

• OOHIRA Fumikazu

  the Department of Intelligent Mechanical Systems Engineering, Faculty of Engineering, Kagawa University

• HASHIGUCHI Gen

  the Department of Intelligent Mechanical Systems Engineering, Faculty of Engineering, Kagawa University

Abstract

In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.

Journal

• IEICE transactions on electronics

  IEICE transactions on electronics 90 (1), 110-115, 2007-01-01

  The Institute of Electronics, Information and Communication Engineers

Keywords

• DNA
• label free
• surface plasmon resonance
• SPR

Details

• CRID: 1572824502369343232
• NII Article ID: 110007519564
• NII Book ID: AA10826283
• ISSN: 09168524
• Text Lang: en
• Data Source:
  • CiNii Articles