1.Temperature and Pressure Effect on Astrocyte Preservation

  • SOTOME Shinichi
    Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University
  • NAKAJIMA Kazuyuki
    Department of Bioinfomatics, Faculty of Engineering, Soka University
  • YOSHIMURA Yukihiro
    Department of Applied Chemistry, National Defense Academy
  • SHIMIZU Akio
    Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University

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  • 1.アストロサイトの保存に対する温度および圧力の影響
  • アストロサイトの保存に対する温度および圧力の影響
  • アストロサイト ノ ホゾン ニ タイスル オンド オヨビ アツリョク ノ エイキョウ

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Abstract

Pressure effect on the survival rate of astrocytes was investigated at room temperature and 4℃. Although tissue and cells are frozen for preservation by adding cryoprotective compound, it is clarified that the survival rate of astrocytes is increased under a pressurized state at room temperature and 4℃. The optimum pressure was 0.3-1.0MPa at both room temperature and 4℃. The prominent effect was observed at 4℃ low temperature. The astrocytes died completely under normal pressure in four days at 4℃. However, under 0.3-1.0MPa, it is clarified that the survival of astrocytes increases dramatically.

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