骨中コラーゲン高親和性タンパク質の骨芽細胞様細胞株MC3T3-E1細胞の骨形成過程に及ぼす影響  [in Japanese] The effect of the collagen-high affinity proteins in bone on the process of bone formation of osteoblastic MC3T3-E1 cells  [in Japanese]

Access this Article

Author(s)

    • 村上 拡治 Murakami Koji
    • 首都大学東京大学院人間健康科学研究科:東京都立保健科学大学大学院保健科学研究科 Graduate School of Human Health Sciences, Tokyo Metropolitan University:Graduate School of Health Sciences, Tokyo Metropolitan University of Health Sciences
    • 細川 健太郎 Hosokawa Kentaro
    • 東京都立保健科学大学大学院保健科学研究科:慶応大学医学部坂口光洋記念講座 Graduate School of Health Sciences, Tokyo Metropolitan University of Health Sciences:The Sakaguchi Laboratory of Developmental Biology, School of Medicine, Keio University
    • 祭 友昭 [他] Sai Tomoaki
    • 東京慈恵会医科大学大学院医学研究科整形外科学講座 Department of Orthopaedic Surgery, Graduate School of Medicine, The Jikei University School of Medicine
    • 笠井 久隆 Kasai Hisataka
    • 首都大学東京大学院人間健康科学研究科:東京都立保健科学大学大学院保健科学研究科 Graduate School of Human Health Sciences, Tokyo Metropolitan University:Graduate School of Health Sciences, Tokyo Metropolitan University of Health Sciences
    • 井上 順雄 Inoue Nobuo
    • 首都大学東京大学院人間健康科学研究科:東京都立保健科学大学大学院保健科学研究科 Graduate School of Human Health Sciences, Tokyo Metropolitan University:Graduate School of Health Sciences, Tokyo Metropolitan University of Health Sciences

Abstract

我々は既に,ウシ大腿骨皮質骨中にコラーゲンに高親和性を持つ,酸性タンパク質を検出,報告した。本研究では,骨芽細胞様細胞株MC3T3-E1細胞の培養系を用いて,このコラーゲン高親和性タンパク質(C-HAP)が骨形成過程に及ぼす影響を検討した。C-HAPを含む内因性のタンパク質精製画分(2画分)と外因性のポリグルタミン酸(PGA)をそれぞれMC3T3-E1細胞の培養系に添加し,細胞形態,ALP活性および骨基質の石灰化を評価した。その結果,(1)PGAはALP活性を増強し,タンパク質の集積と骨基質の石灰化を促進したこと,(2)C-HAPはALP活性を減弱し,タンパク質の集積と骨基質の石灰化を促進したこと,また,(3)PGAが細胞を多角形に保つのに対して,C-HAPは細胞を紡錘形に変えたことが明らかとなった。以上の結果を踏まえて,骨中のコラーゲン高親和性タンパク質の骨形成過程における役割を考察した。

We have reported several acidic noncollagenous proteins in the acetic acid extract of bovine femoral cortical bone, characteristic of their acidity and high affinity to collagen. In this study, we investigated effects of the collagen-high affinity proteins (C-HAP) on the process of bone formation using osteoblast-like MC3T3-E1 cells. To the calcification-promoting medium for MC3T3-E1 cells were added the excess amount of endogenous protein fractions containing C-HAP or exogenous polyglutamic acid (PGA). The process of bone formation of MC3T3-E1 cells were evaluated by the change of cell shape, ALP activity, accumulation of protein and calcification of bone matrix. Thus, it concluded that (1) PGA stimulated ALP activity, accumulation of protein and calcification of bone matrix, (2) C-HAP inhibited ALP activity but stimulated accumulation of protein and calcification of bone matrix, and (3) PGA maintained a polygonal shape in cell structure and C-HAP modulated a cells to a spindle shape. We discussed also whether the collagen-high affinity proteins may play some important roles in the calcification process of bone formation.

Journal

  • The Journal of Japan Academy of Health Sciences

    The Journal of Japan Academy of Health Sciences 12(4), 237-244, 2010

    Japan Academy of Health Sciences

References:  19

Cited by:  1

Codes

  • NII Article ID (NAID)
    110007593174
  • NII NACSIS-CAT ID (NCID)
    AA12001334
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    1880-0211
  • NDL Article ID
    10644508
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z74-B494
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
Page Top