浸透圧変化によるモルモット気道上皮イオントランスポートの反応性の検討  [in Japanese] EFFECT OF OSMOLALITY ON ION TRANSPORT IN GUINEA PIG AIRWAY EPITHELIAL CELLS  [in Japanese]

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Author(s)

    • 服部 知洋 Hattori Tomohiro
    • 日本大学医学部内科学系呼吸器内科学分野 Division of Respiratory Medicine, Department of Medicine, Nihon University School of Medicine
    • 馬島 徹 Majima Toru
    • 国際医療福祉大学臨床医学センター化学療法研究所附属病院呼吸器内科 International University of Health and Welfare, Chemotherapy Institute, Kaken Hospital, Respiratory Center
    • 橋本 修 Hashimoto Shu
    • 日本大学医学部内科学系呼吸器内科学分野 Division of Respiratory Medicine, Department of Medicine, Nihon University School of Medicine

Abstract

【背景】運動誘発喘息(Exercise-induced asthma,EIA)の誘因に,過換気に伴う水分喪失による気道液浸透圧の上昇が考えられている.【目的】モルモット気道上皮イオントランスポートの浸透圧変化による反応性を検討した.【方法】高張食塩水及びマンニトール負荷による影響をopen circuit potential differenceを測定することにより検討した.【結果】0.9-10.8%粘膜側高張食塩水負荷で,diphenylamine-2-carboxylate(DPC),amiloride,disodium cromoglycate(DSCG)はpotential difference(PD)の増加を抑制した.1.8%粘膜側高張食塩水単独負荷でPDの増加を認めたが,585mOsm/kgH_2O粘膜側マンニトール単独負荷では増加を認めず,Cl-free溶液でも同様であった.1.8%粘膜側高張食塩水単独負荷後の組織学的検討では,DPCでのみ気道上皮の厚みの減少を有意に抑制していた.【結語】PDの上昇には,気道液中の浸透圧変化だけでなく,Cl^-,Na^+濃度の変化も重要と考えられた.またcyclic-AMP dependent Cl^-チャンネルの関与が推測され,EIAの病態にも同様なことが示唆されると考えられた.

Background: Increased osmolality of the airway surface fluid due to water loss associated with hyperventilation is considered to be a cause of exercise-induced asthma (EIA). Aim: We investigated the influence of changes in osmolality on airway epithelial ion transport in guinea pigs. Methods: We explored from measuring open circuit potential difference in challenge with hypertonic saline or mannitol. Results: Diphenylamine-2-carboxylate (DPC), amiloride, and disodium cromoglycate (DSCG) prevented an increase of the potential difference (PD) after exposure of tracheal mucosa to 0.9-10.8% hypertonic saline solutions (HSSs) (p<0.05, p<0.01, and p<0.01, respectively on 1.8%). An increase of the PD was observed after a single dose of 1.8% hypertonic saline solution (HSS) was applied to the mucosa, but no increase was observed after a single exposure to a 585mOsm/kg aqueous mannitol solution (585AMS). The results remained the same when a Cl-free solution was used as the perfusate. The change of airway epithelial cell thickness was only suppressed significantly by DPC (p<0.01) after 1.8% HSS challenge. Conclusions: Not only the change of osmolality, but also changes of the Cl^- and Na^+ concentrations in airway surface fluid seem to have an important influence on the PD. cAMP-dependent Cl^- channel may have a role, and the same mechanism may provoke EIA.

Journal

  • Japanese Journal of Allergology

    Japanese Journal of Allergology 59(8), 974-983, 2010

    Japanese Society of Allergology

References:  15

Codes

  • NII Article ID (NAID)
    110007700754
  • NII NACSIS-CAT ID (NCID)
    AN00012583
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0021-4884
  • NDL Article ID
    10834171
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z19-32
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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