ハイドロキシアパタイトに対する新規リン酸エステル塩型ハイブリッド界面活性剤の改質効果  [in Japanese] Modification Effect of Novel Phosphate-type Hybrid Surfactant on Hydroxyapatite  [in Japanese]

Access this Article

Search this Article

Author(s)

    • 森 梨江 MORI Rie
    • 神奈川歯科大学口腔治療学講座保存修復学分野 Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College
    • 二瓶 智太郎 NIHEI Tomotaro
    • 神奈川歯科大学口腔治療学講座保存修復学分野 Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College
    • 大橋 桂 OHASHI Katsura
    • 神奈川歯科大学口腔治療学講座保存修復学分野 Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College
    • 寺中 敏夫 TERANAKA Toshio
    • 神奈川歯科大学口腔治療学講座保存修復学分野 Division of Restorative Dentistry, Department of Oral Medicine, Kanagawa Dental College

Abstract

水に易溶性で歯磨剤に添加可能な1分子内にカルシウムと結合するリン酸エステル塩基と,フッ化炭素鎖および炭化水素鎖を有するハイブリッド界面活性剤であるsodium phenyl 1-[(4-perfluorohexyl)phenyl]-1-hexylphosphate(F6H5OP)を新規に合成した.F6H5OPで処理したハイドロキシアパタイト焼結体(HAP pellet)ならびにハイドロキシアパタイト粉末(HAP powder)を用い,表面自由エネルギーおよびタンパク質の吸着抑制能を測定し,プラーク形成抑制効果を基礎的に検討した.表面自由エネルギーは,HAP pelletを1.0〜5.0mmol/lのF6H5OP水溶液で処理後,直後および37℃水中に3,12時間保管後に測定した.HAP powderに対するF6H5OPの吸着量は,F6H5OP水溶液にHAP powderを加えて5〜60分反応後高速液体クロマトグラフィーにて測定した.タンパク質の吸着抑制効果は,F6H5OP水溶液でそれぞれ改質したHAP powderを1,000μg/2mlのホスビチンに加えて10分〜12時間反応させ,ゲル浸透クロマトグラフィーにより測定した.また,改質HAP powderの水による洗浄に対する耐久性と,洗浄後のホスビチン吸着抑制効果についても検討した.F6H5OPで改質されたHAP pelletの表面自由エネルギーは水中保管12時間においてもコントロールと比較して有意に低い値であった.HAP powderへのF6H5OPの吸着量は,改質開始5分で70〜80%,60分後には80〜95%であった.改質HAP powderに対するホスビチンの吸着量は,2時間後で146〜276μgとコントロールと比較して有意に低い値を示した.また,改質HAP powder 2回洗浄後のホスビチン吸着量は反応12時間後も約40%の抑制を受けた.以上の結果より,F6H5OPによるハイドロキシアパタイトの改質は,表面に撥水性および撥油性,ならびにホスビチンの吸着抑制効果を12時間以上にわたって維持し,プラークの歯面付着を抑制しうることが示唆された.

It has been suggested that reducing the surface free energy of the tooth surface may prevent plaque accumulation and reduce the incidence of caries and periodontal disease. We synthesized a novel aqueous hybrid surfactant that can be added to dentifrice, namely sodium phenyl 1-[(4-perfluorohexyl)phenyl]-1-hexylphosphate (F6H5OP), which is a molecule containing a long fluorocarbon chain, a hydrocarbon chain, and a phosphate ester. In this study, we investigated the reduction in surface free energy on a hydroxyapatite (HAP) pellet and the inhibition of the calcium binding protein, phosvitin, on HAP powder modified with F6H5OP The amount of F6H5OP adsorbed to the HAP powder was determined by high-performance liquid chromatography. The F6H5OP water solution was applied to HAP pellets with a microbrush. The specimens were stored in water for 3 and 12 hours at 37℃. The surface free energies of the modified specimens were calculated by measuring the surface contact angles of distilled water and diiodomethan. The amount of phosvitin adsorbed to the HAP powder was determined by gel permeation chromatography. The resistance to rinsing with deionized water and the inhibitory effect of phosvitin adsorption to HAP powder were also measured. More than 70% of F6H5OP in deionized water was adsorbed to the HAP powder within 5 minutes. The contact angles of the HAP pellets modified with F6H5OP were significantly higher than that of the non-treated control, and the surface free energies were significantly lower than that of the control after the 12-hour water immersion. The inhibition ratio of phosvitin adsorption to modified HAP powder following rinsing twice with deionized water was about 40% after immersion in water for 12 hours. These results suggest that the novel phosphate-type hybrid surfactant F6H5OP has a significant modification effect on the surface of hydroxyapatite.

Journal

  • The Japanese Journal of Conservative Dentistry

    The Japanese Journal of Conservative Dentistry 53(2), 182-190, 2010

    The Japanese Society of Conservative Dentistry

References:  25

Cited by:  1

Codes

  • NII Article ID (NAID)
    110007880724
  • NII NACSIS-CAT ID (NCID)
    AN00191201
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0387-2343
  • Data Source
    CJP  CJPref  NII-ELS  J-STAGE 
Page Top