Effect of IL-6 on Expression of FGF-2 in Human Dental Pulp Cells

• FUJIWARA Hideaki

  Division of Periodontology, Department of Conservative Dentistry and Oral Rehabilitation, School of Dentistry, Iwate Medical University

• YAEGASHI Takashi

  Division of Periodontology, Department of Conservative Dentistry and Oral Rehabilitation, School of Dentistry, Iwate Medical University

• HATAKEYAMA Setsuko

  Division of Oral Pathology, Department of Pathogenesis and Control of Oral Diseases, School of Dentistry, Iwate Medical University

• TAKEDA Yasunori

  Division of Oral Pathology, Department of Pathogenesis and Control of Oral Diseases, School of Dentistry, Iwate Medical University

• ISHIHIRA Youji

  Ishihira Dental Office

• KUNIMATSU Kazushi

  Division of Periodontology, Department of Conservative Dentistry and Oral Rehabilitation, School of Dentistry, Iwate Medical University

Bibliographic Information

Other Title

• IL-6が培養歯髄由来細胞のFGF-2発現に与える影響

Abstract

Interleukin (IL)-6 is a major mediator in a host response against tissue injury and bacterial infection. It is highly released by macrophage, fibroblast and endothelial cells in the inflammatory regions. Recently, it has been studied that the fibroblast growth factor (FGF)-2 is used in clinical regeneration of dentin-pulp complex lost due to dental caries or trauma. The purpose of this study was to investigate the effect of IL-6 on the expression of FGF-2 in human dental pulp cells. Dental pulp cells were cultured in the medium with IL-6 at a concentration of 0, 0.1, 1, 10ng/ml for 3 weeks. The expression of FGF-2 in the cells was examined by the method of a real-time PCR. Statistical analysis was done by one-way ANOVA. The localization of FGF-2 in dental pulp cells was also examined by immunohistochemical staining. As a result, a real-time PCR analysis revealed that IL-6 at a concentration of 1 and 10ng/ml inhibited the expression of FGF-2 mRNA in the cultured cells and immunohistochemical staining demonstrated that IL-6 clearly decreased the immunoreaction against FGF-2 in the dental pulp cells. These results indicated that IL-6 inhibited the FGF-2 expression in dental pulp cells.

Journal

• The Japanese Journal of Conservative Dentistry

  The Japanese Journal of Conservative Dentistry 53 (2), 207-213, 2010

  The Japanese Society of Conservative Dentistry

Keywords

• Dental pulp cell
• IL-6
• FGF-2

Details

• CRID: 1390001205520338432
• NII Article ID: 110007880726
• NII Book ID: AN00191201
• DOI: 10.11471/shikahozon.53.207
• ISSN: 21880808
• ISSN: 03872343
• Web Site: https://search.jamas.or.jp/link/ui/2010226866
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed