Synergistic anti-tumor effect of CDDP with CRM197, a specific inhibitor of HB-EGF, in oral cancer

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著者

    • Dateoka Suguru
    • Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University
    • Ohnishi Yuichi
    • Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
    • Kakudo Kenji
    • Second Department of Oral and Maxillofacial Surgery, Osaka Dental University

抄録

CRM197, a mutated diphtheria toxin (DT), has been found in nontoxic proteins. Because it is non-toxic, this protein has been utilized for various purposes, including as an inhibitor of HB-EGF, which belongs to the EGF family, and has been implicated in the increased progression, proliferation, and metastasis of oral cancer. We analyzed the anti-tumor effects of CRM197 in oral cancer. Inhibitory agents against HB-EGF, such as CRM197, represent possible chemotherapeutic and chemosensitizing agents for oral cancer. In this experiment, we used the oral squamous cell carcinoma cell line HSC3, HSC4, and SAS. Cells treated with CRM197 were analyzed based on cell viability, MTT assay, Western blot, and zymography, following the manufacturers' instructions. Cell proliferation on treatment with CRM197 and/or CDDP was inhibited and apoptosis was observed. The invasiveness of CRM197-treated oral squamous cell carcinoma cells was relatively low. MMP-9 expression on reverse transcription polymerase chain reaction (RT-PCR) revealed that MMP-9 was suppressed in oral squamous cell carcinoma cells treated with CRM197. These results suggest that HB-EGF is a target for cancer therapy. We found that not only is CRM197 effective as a specific inhibitor of HB-EGF, but it also has a synergistic anti-tumor effect when combined with CDDP in oral cancer therapy.

収録刊行物

  • Journal of Osaka Dental University

    Journal of Osaka Dental University 45(1), 31-36, 2011

    大阪歯科学会

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各種コード

  • NII論文ID(NAID)
    110008607465
  • NII書誌ID(NCID)
    AA00704144
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0475-2058
  • データ提供元
    CJP書誌  NII-ELS  J-STAGE 
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