書誌事項
- タイトル別名
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- Cryopreservation of Nematode Caenorhabditis elegans in the Adult Stage
- センチュウ Caenorhabditis elegans ノ セイチュウ ステージ ニ オケル トウケツ ホゾン
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Cryopreservation of the nematode Caenorhabditis elegans in the adult stage is of importance as the nematode is a powerful model organism. In this study, we applied the protocol previously established for cryopreservation of the L4 nematode to the adult one. Survival rate of 84% was obtained as a result. When ice nucleation active bacteria, Pseudomonas syringae, was used for the simplification of ice seeding procedure instead of the use of a pre-cooled needle, survival rate of 82% was obtained after thawing. Moreover, a simple method of cryopreservation using a deep freezer and polystyrene foam was developed for a practical purpose. According to the observation under a cryomicroscope, eggs in the nematode body were found to freeze at ca. -20℃ when cooled at the rate of 0.2℃/min after ice seeding at -3℃ although the nematode body remained unfrozen down to -100℃.
収録刊行物
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- 低温生物工学会誌
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低温生物工学会誌 57 (1), 87-90, 2011
低温生物工学会
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詳細情報 詳細情報について
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- CRID
- 1390001205087189760
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- NII論文ID
- 110008671128
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- NII書誌ID
- AN10448734
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- ISSN
- 24241555
- 13407902
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- NDL書誌ID
- 11094651
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可