Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor

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Author(s)

    • ZHENG Yun-wen
    • Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University
    • UENO Yasuharu
    • Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University
    • KOIKE Naoto
    • Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University
    • TANAKA Junzo
    • Regeneration Materials Group, Biomaterials Center, National Institute for Materials Science
    • TANIGUCHI Hideki
    • Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University

Abstract

Reconstitution of tissue architecture in vitro is important because it enables researchers to investigate the interactions and mutual relationships between cells and cellular signals involved in the three-dimensional (3D) construction of tissues. To date, in vitro methods for producing tissues with highly ordered structure and high levels of function have met with limited success although a variety of 3D culture systems have been investigated. In this study, we reconstituted functional hepatic tissue including mature hepatocyte and blood vessel-like structures accompanied with bile duct-like structures from E15.5 fetal liver cells, which contained more hepatic stem/progenitor cells comparing with neonatal liver cells. The culture was performed in a simulated microgravity environment produced by a rotating wall vessel (RWV) bioreactor. The hepatocytes in the reconstituted 3D tissue were found to be capable of producing albumin and storing glycogen. Additionally, bile canaliculi between hepatocytes, characteristics of adult hepatocyte in vivo were also formed. Apart from this, bile duct structure secreting mucin was shown to form complicated tubular branches. Furthermore, gene expression analysis by semiquantitative RT-PCR revealed the elevated levels of mature hepatocyte markers as well as genes with the hepatic function. With RWV culture system, we could produce functionally reconstituted liver tissue and this might be useful in pharmaceutical industry including drug screening and testing and other applications such as an alternative approach to experimental animals.

Journal

  • Journal of bioscience and bioengineering

    Journal of bioscience and bioengineering 111(6), 711-718, 2011-06-25

    The Society for Biotechnology, Japan

References:  25

Cited by:  1

Codes

  • NII Article ID (NAID)
    110008672821
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13891723
  • NDL Article ID
    11137003
  • NDL Source Classification
    ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
  • NDL Call No.
    Z53-S65
  • Data Source
    CJP  CJPref  NDL  NII-ELS  Crossref 
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