シイタケのホモタリック変異菌株の担子胞子形成場面における核行動 Nuclear behavior during basidiospore formation in a homothallic mutant of Lentinula edodes

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Abstract

シイタケのホモタリック変異菌株の担子胞子形成場面における核行動を解析した.塩酸ギムザ染色したヒダ組織を光学顕微鏡で観察したところ,核融合と減数分裂が正常に行われ,減数分裂後に小柄が形成されることが判明した.四分子核の体細胞分裂が担子胞子において一度生じた.また,非同調の核移動を示唆する担子器が認められた.担子胞子を4',6-diamidino-2-phenylindole dihydrochloride(DAPI)染色して蛍光顕微鏡で観察したところ,野生型のシイタケ菌株では90%以上が1核の担子胞子であったが,変異菌株では核数に異常が認められた.すなわち,半数近くがDAPI陰性であり,約30%が1核,そして約10%が2核であった.第一分裂前期核を電子顕微鏡で観察したところ,3つの要素で構成されている典型的なシナプトネマ複合体が存在することが判明した.

Nuclear behavior during basidiospore formation in a homothallic mutant of Lentinula edodes was examined. Light microscopic observations of gill tissues after HC1-Giemsa staining revealed that karyogamy and meiosis were completed normally and sterigmata developed after meiosis. In basidia bearing basidiospore initials, a single post-meiotic mitotic division of the tetrads occurred in each basidiospore. Unsynchronized nuclear migration via sterigma was evident in the binucleate basidium bearing three binucleate basidiospores. Microscopic observations of basidiospores stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) revealed that more than 90% of the basidiospores of wild-type strains were uninucleate, but the mutant produced basidiospores carrying an irregular number of nuclei; about half of the basidiospores were DAPI-negative, about 30% were uninucleate and approximately 10% were binucleate. Electron microscopic observation of prophase I nucleus revealed the existence of typical synaptonemal complexes composed of three elements.

Journal

  • Mushroom Science and Biotechnology

    Mushroom Science and Biotechnology 19(2), 88-92, 2011

    Japanese Society of Mushroom Science and Biotechnology

References:  14

Codes

  • NII Article ID (NAID)
    110008711283
  • NII NACSIS-CAT ID (NCID)
    AA12415767
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    1348-7388
  • NDL Article ID
    11209441
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-B266
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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