Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system
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- Ochiai Hiroshi OCHIAI Hiroshi
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Harashima Hideyoshi HARASHIMA Hideyoshi
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Kamiya Hiroyuki KAMIYA Hiroyuki
- Faculty of Pharmaceutical Sciences, Hokkaido University
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Author(s)
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- Ochiai Hiroshi OCHIAI Hiroshi
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Harashima Hideyoshi HARASHIMA Hideyoshi
- Faculty of Pharmaceutical Sciences, Hokkaido University
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- Kamiya Hiroyuki KAMIYA Hiroyuki
- Faculty of Pharmaceutical Sciences, Hokkaido University
Abstract
The effects of a genetic insulator (chicken cHS4) on transgene expression in a positive feedback system using the GAL4-VP16 activator and luciferase reporter plasmids were examined. The introduction of cHS4 enhanced luciferase expression in mouse liver, indicating that this system would be useful for efficient transgene expression.
Journal
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- Journal of bioscience and bioengineering
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Journal of bioscience and bioengineering 112(5), 432-434, 2011-11-25
Elsevier B.V.
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