Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1
-
- Morohoshi Tomohiro MOROHOSHI Tomohiro
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
-
- Ogata Yuji OGATA Yuji
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
-
- Ikeda Tsukasa IKEDA Tsukasa
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
Access this Article
Search this Article
Author(s)
-
- Morohoshi Tomohiro MOROHOSHI Tomohiro
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
-
- Ogata Yuji OGATA Yuji
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
-
- Ikeda Tsukasa IKEDA Tsukasa
- Department of Material and Environmental Chemistry, Graduate School of Engineering, Utsunomiya University
Abstract
Pantoea ananatis SK-1 produces N-acylhomoserine lactones (AHLs) and regulates expression of some virulence factors through AHL-mediated quorum sensing. In this study, we discovered that the strain SK-021, which has a disrupted AHL-synthetic gene, showed constitutive cell aggregation. SK-1 has the ability to aggregate, and cell aggregation inhibitory factors are expressed under control of AHL-mediated quorum sensing. One of the transposon mutants, SK-33M, constitutively aggregates without defective AHL production. A homology search revealed that the transposon integration site was located in the adhesin-like yeeJ gene. Based on RT-PCR analysis, transcription of yeeJ is regulated by AHL-mediated quorum sensing. However, because both the wild-type and SK-33M strains induced necrotic symptoms in onion leaves, we conclude that the yeeJ gene is not involved in the pathogenicity of SK-1.
Journal
-
- Journal of bioscience and bioengineering
-
Journal of bioscience and bioengineering 112(6), 566-569, 2011-12-25
The Society for Biotechnology, Japan
References: 19
-
1
- Quorum sensing in plant pathogenic bacteria
-
VON BODMAN S. B.
Annu. Rev. Phytopathol. 41, 455-482, 2003
Cited by (1)
-
2
- Pantoea ananatis : an unconventional plant pathogen
-
COUTINHO T. A.
Mol. Plant Pathol. 10, 325-335, 2009
Cited by (1)
-
3
- <no title>
-
SAMBROOK J.
Molecular cloning : a laboratory manual, 1989
Cited by (1)
-
4
- Genome sequence of Pantoea ananatis LMG20103, the causative agent of Eucalyptus blight and dieback
-
DE MAAYER P. W.
J. Bacteriol. 192, 2936-2937, 2010
Cited by (1)
-
5
- Coaggregation between Acinetobacter johnsonii S35 and Microbacterium esteraromaticum Strains Isolated from Sewage Activated Sludge
-
MALIK ANUSHREE , SAKAMOTO MASASHI , ONO TAISHIRO , KAKII KAZUO
Journal of Bioscience and Bioengineering 96(1), 10-15, 2003-07-25
References (15) Cited by (2)
-
6
- Bacterial quorum sensing in pathogenic relationships
-
DE KIEVIT TR
Infect. Immun. 68, 4839-4849, 2000
DOI Cited by (17)
-
7
- Quorum-sensing in Gram-negative bacteria
-
WHITEHEAD NA
FEMS Microbiol. Rev. 25, 365-404, 2001
DOI Cited by (15)
-
8
- Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone
-
CHATTERJEE A
Appl. Environ. Microbiol. 61, 1959-1967, 1995
Cited by (5)
-
9
- Capsular polysaccharide biosynthesis and pathogenicity in Erwinia stewartii require induction by an N-acylhomoserine lactone autoinducer
-
VON BODMAN SB
J. Bacteriol. 177, 5000-5008, 1995
Cited by (7)
-
10
- The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing
-
MOROHOSHI T.
J. Bacteriol. 189, 8333-8338, 2007
Cited by (8)
-
11
- Synthetic analogues of the bacterial signal (quorum sensing) molecule N-(3-oxododecanoyl)-L-homoserine lactone as immune modulators
-
CHHABRA S. R.
J. Med. Chem. 46, 97-104, 2003
DOI Cited by (6)
-
12
- Mutagenesis of Burkholderia pseudomallei with Tn5-OT182 : isolation of motility mutants and molecular characterization of the flagellin structural gene
-
DESHAZER D.
J. Bacteriol. 179, 2116-2125, 1997
Cited by (4)
-
13
- Construction and use of a self-cloning promoter probe vector for gram-negative bacteria
-
MERRIMAN TR
Gene 126, 17-23, 1993
Cited by (2)
-
14
- Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA
-
MARCHESI JR
Appl Environ Microb 64, 795-799, 1998
Cited by (23)
-
15
- FimH-mediated autoaggregation of Escherichia coli
-
SCHEMBRI M. A.
Mol. Microbiol. 41, 1419-1430, 2001
DOI Cited by (2)
-
16
- Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli : cell aggregation and biofilm formation
-
SHERLOCK O.
J. Bacteriol. 186, 8058-8065, 2004
Cited by (2)
-
17
- Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation
-
CHANDLER J. R.
J. Bacteriol. 191, 5901-5909, 2009
Cited by (1)
-
18
- A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides
-
PUSKAS A
J. Bacteriol. 179, 7530-7537, 1997
Cited by (3)
-
19
- Exopolysaccharide Production Is Influenced by Sugars, N-Acylhomoserine Lactone, and Transcriptional Regulators RcsA and RcsB, but Does Not Affect Pathogenicity in the Plant Pathogen Pantoea ananatis
-
MOROHOSHI Tomohiro , OSEKI Kiemi , IKEDA Tsukasa
Bioscience, Biotechnology, and Biochemistry 75(5), 997-999, 2011-05-23
J-STAGE References (16) Cited by (1)