Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1

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Abstract

Pantoea ananatis SK-1 produces N-acylhomoserine lactones (AHLs) and regulates expression of some virulence factors through AHL-mediated quorum sensing. In this study, we discovered that the strain SK-021, which has a disrupted AHL-synthetic gene, showed constitutive cell aggregation. SK-1 has the ability to aggregate, and cell aggregation inhibitory factors are expressed under control of AHL-mediated quorum sensing. One of the transposon mutants, SK-33M, constitutively aggregates without defective AHL production. A homology search revealed that the transposon integration site was located in the adhesin-like yeeJ gene. Based on RT-PCR analysis, transcription of yeeJ is regulated by AHL-mediated quorum sensing. However, because both the wild-type and SK-33M strains induced necrotic symptoms in onion leaves, we conclude that the yeeJ gene is not involved in the pathogenicity of SK-1.

Journal

  • Journal of bioscience and bioengineering

    Journal of bioscience and bioengineering 112(6), 566-569, 2011-12-25

    The Society for Biotechnology, Japan

References:  19

Codes

  • NII Article ID (NAID)
    110008897330
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13891723
  • NDL Article ID
    023359475
  • NDL Call No.
    Z53-S65
  • Data Source
    CJP  NDL  NII-ELS  Crossref 
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