30. 5-DeoxystrigolおよびGR24をリガンドとする光親和性プローブの合成と活性評価(口頭発表,植物化学調節学会第46回大会)
書誌事項
- タイトル別名
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- 30. Synthesis and biological evaluation of phenyldiazirine-containing 5-deoxystrigol and GR24 photoaffinity probes for isolation of strigolactone receptor in AM fungi
抄録
Arbuscular mycorrhizal fungi (AM fungi) form mutualistic, symbiotic associations with more than 80% of land plants. In one of the first stages of host recognition, the hyphae of AM fungi show extensive branching by recognizing strigolactones (SLs) exuded from host plant roots. Our extensive structure-activity relationship study demonstrated that SLs show potent activity at very low concentrations in a structure-dependent manner, suggesting that the induction of hyphal branching proceeds via a receptor-mediated mechanism. However, the AM fungal SL receptor has not been isolated. Here, we designed and synthesized phenyldiazirine-containing 5-deoxystrigol (5DS) and GR24 probes for photoaffinity labeling of SL receptor in AM fungi. An alkynyl phenyldiazirine photophore was respectively introduced at C-5 of 5DS and at C-7 of GR24. The hyphal branching activities of 5DS photoaffinity probes were much weaker than those of 5DSs (1-10 ng/disc vs 3-30 pg/disc), while the GR24 photoaffinity probes showed comparable activity to GR24 (1 ng/disc vs 1 ng/disc).
収録刊行物
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- 植物化学調節学会 研究発表記録集
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植物化学調節学会 研究発表記録集 46 (0), 46-, 2011
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390001204422058240
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- NII論文ID
- 110008902439
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- ISSN
- 21896313
- 09191887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 使用不可