31. AM菌のストリゴラクトン受容体単離のための高活性型光親和性プローブの合成(口頭発表,植物化学調節学会第46回大会)

• 秋山 康紀

  大阪府大院・生命環境・応用生命科学

• 中谷 成史

  大阪府大院・生命環境・応用生命科学

• 林 英雄

  大阪府大院・生命環境・応用生命科学

書誌事項

タイトル別名

• 31. Design and development of highly active photoaffinity probes for isolation of strigolactone receptor in AM fungi

抄録

Arbuscular mycorrhizas formed between more than 80% of land plants and arbuscular mycorrhizal (AM) fungi belonging to the Glomeromycota are the most common and widespread symbiosis on our planet. Strigolactones (SLs) exuded from host plant roots act as a host-recognition signal for AM fungi. Very low concentrations of SLs can induce the host recognition response, hyphal branching, in AM fungi. This suggests a highly sensitive perception system for SLs present in AM fungi. However, very little is known about the molecular mechanisms underlying SL perception and induction of hyphal branching in AM fungi. We have recently started to design and synthesize molecular probes for photoaffinity labeling of SL receptor proteins in AM fungi. In this study, we modified ligand SL structure to increase sensitivity and specificity in photoaffinity labeling. 4a-Hydroxy-GR24 synthesized from 1,3-indandione showed comparable activity to GR24 in an AM fungus Gigaspora margarita (100 pg/disc). 8-Desmethylsorgolactone (8-dMS) synthesized from cyclohexanone exhibited strong but slightly weaker activity than 5-deoxystrigol (5DS) (10 pg/disc vs 3 pg/disc). This suggests that the introduction of functionality at C-8 could increase hyphal branching activity. Thus, we are now synthesizing 8-dMS photoaffinity probes that possess a phenyldiazirine photophore at C-8.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 46 (0), 47-, 2011

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390282679398767232
• NII論文ID: 110008902440
• DOI: 10.18978/jscrpanb.46.0_47
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可