アミノ酸の窒素同位体比を用いた水棲生物の栄養段階の解析  [in Japanese] Food chain analysis by nitrogen isotopic composition of amino acids  [in Japanese]

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Author(s)

    • 力石 嘉人 CHIKARAISHI Yoshito
    • 独立行政法人海洋研究開発機構海洋・極限環境生物圏領域 Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • 小川 奈々子 OGAWA Nanako O.
    • 独立行政法人海洋研究開発機構海洋・極限環境生物圏領域 Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • 高野 淑識 [他] TAKANO Yoshinori
    • 独立行政法人海洋研究開発機構海洋・極限環境生物圏領域 Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • 土屋 正史 TSUCHIYA Masashi
    • 独立行政法人海洋研究開発機構海洋・極限環境生物圏領域 Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
    • 大河内 直彦 OHKOUCHI Naohiko
    • 独立行政法人海洋研究開発機構海洋・極限環境生物圏領域 Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)

Abstract

Nitrogen isotopic composition (δ<sup>15</sup>N) of individual amino acids has recently been employed as a potential powerful method for estimating the trophic level of organisms in food webs. In metabolic processes, one group of amino acids has little change in their nitrogen isotopic composition (e.g., 0.4‰ for phenylalanine), although another group has a large isotopic fractionation (e.g., 8.0‰ for glutamic acid). This fractionation could be associated with the cleavage of carbon-nitrogen bond in the metabolic processes (e.g., transamination) of amino acids. Therefore, a comparison between δ<sup>15</sup>N values of these two types of amino acids would provide the trophic level of organisms. In fact, we can estimate the trophic levels of aquatic organisms with a small error (1σ = 0.12), employing the equation: [Trophic level] = (δ<sup>15</sup>N<sub>glutamic acid</sub>-δ<sup>15</sup>N<sub>phenylalanine</sub>-3.4)/7.6+1. Thus, a key advantage of this method is that the trophic level can be obtained based on the δ<sup>15</sup>N values of two amino acids from a single organism; consequently, unlike the bulk method, it is not necessary to characterize the δ<sup>15</sup>N values of primary producers. Here, we review the principle of this amino acid method and its application to natural organisms in marine and freshwater environments.

Journal

  • Chikyukagaku

    Chikyukagaku 44(4), 233-241, 2010

    The Geochemical Society of Japan

References:  44

Cited by:  2

Codes

  • NII Article ID (NAID)
    110009327698
  • NII NACSIS-CAT ID (NCID)
    AN00141280
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0386-4073
  • NDL Article ID
    10976440
  • NDL Source Classification
    ZM41(科学技術--地球科学)
  • NDL Call No.
    Z15-645
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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